Historically, milk tankers were cleaned after every load. Consolidation of the industry has led to longer routes and use of tankers for up to 24 h in between cleans. This study focused on the effect of frequent tanker use hauling on raw milk quality. Standard tanker use, (CIP once per 24 h) served as our control and incremental cleaning treatments (water rinse after each load, water rinse after each load with a 12 h sanitizer treatment, and 12 h sanitizer treatment) were added to the study to understand if any effect could be mitigated by more frequent cleans. Two trucks were isolated for this study, which utilized a mix model with repeated measures design. Each truck was utilized for up to 9 routes per day. To understand the effect of seasonality, the 8-d study was repeated in both summer and winter. Producer samples were collected from the farm bulk tank before loading raw milk into the tanker as well as sampling the same milk directly out of the tanker truck before unloading at the manufacturer. Milk quality was quantified through commonly utilized microbiological tests: total bacteria count, thermophilic spore count and preliminary incubation count. Within our study, we defined a negative effect on milk quality as a statistically significant (P < 0.05) difference between the producer and tanker sample in any of the 3 microbial tests conducted. Results did not identify a negative effect on raw milk quality due to hauling neither in summer nor in winter conditions. Therefore, the addition of cleaning treatments did not appear to provide a positive impact on milk quality. Based on this study, current practices appear to be effective in mitigating any measurable negative effect due to hauling.