The majority of the mesophilic bacteria found in raw milk, has a big involvement in the refrigerated milk degradation and its dairy products producing enzymes with proteolytic and lipolytic action. This research was conducted in the state of west region of Parana- Brazil. The milk samples from 35 properties were collected directly from the cooling tanks and analyzed by flow cytometry for TBC and SCC. The proteolytic and lipolytic microorganisms were plated on caseinate agar and tributyrin agar both incubated at 35°C for 48 h. For all milk samples was also evaluated in the presence of Staphylococcus spp. at 3 critical points of production: from milking, hands-of-milkers, milk cooling tank and plated on Baird Parker agar being held coagulase test. It was possible to stratify the milk samples into 4 distinct treatments according to the values of somatic cells count and total bacteria count: Treatment 1 (>600.000 cells/mL), Treatment 2 (>600.000 cfu/mL), Treatment 3 (>600.000 cells/mL and cfu/mL) and Treatment 4 (<600.000 cells/mL and cfu/mL). The treatments showed mean values of proteolytic microorganisms: 2.7 × 10³ cfu/mL, 1.8 × 10⁴ cfu/mL, 1.9 × 10⁴ cfu/mL, 1.4 × 10⁴ cfu/mL and lipolytic microorganisms 1.0 × 10⁵ cfu/mL, 2.1 × 10⁵ cfu/mL, 2.0 × 10⁵ cfu/mL, 6.2 × 10⁴ cfu/mL, with no significant difference by Tukey test (P > 0.05). The dairy production systems had unsatisfactory hygienic conditions. Moderate contamination were obtained from Staphylococcus 9.6 × 10³ cfu/mL, 2.2 × 10³ cfu/mL, 1.4 × 10⁴ cfu/mL and 3.8 × 10³ cfu/mL cfu/mL; for milking, hands-of-milker, cooling tank and milk respectively. The results show the presence of Staphylococcus agent in dairy production systems.