Bisphenol A (BPA) is a widely used monomer of polycarbonate plastics and epoxide resin that has been implicated in allergy related disease. Allergy is an abnormal immune response to an allergen. Type I hypersensitivity is an immunoglobulin (Ig) E-mediated allergic disorder. This study investigated the inhibitory effect of the Lactobacillus plantarum L67 extract on BPA induced allergic inflammatory response in RBL-2H3 cells. RBL-2H3 cells were treated with bisphenol A (50 μM/mL) and co-treated with the L. plantarum L67 extract (5–100 μg/mL) for 30 min. histamine releasing, β-hexosaminidase and intracellular Ca²⁺ level were estimated in the medium. IgE production and activities of PKC, iNOS were measured ELISA and Western blotting separately. Also IL-4 and TNF-α were measured by RTq-PCR. The histamine releasing of treatment with the L. plantarum L67 extract considerably decreased in a concentration dependent manner. When the cells were treated with the 100 μg/mL L. plantarum L67 extract, the values of histamine releasing decreased 0.48-fold compared with the BPA treatment alone. The addition of BPA caused a 1.43-fold increase in IgE levels compared with control, while the values of IgE amounts decreased by 1.31, 1.28, 1.14, 1.08 and 1.04 at addition L. plantarum L67 extract (5–100 μg/mL) in the presence of BPA, compared with that for the BPA treatment alone. Our results showed that L. plantarum L67 extract inhibited the histamine releasing, β-hexosaminidase, productions of IgE, the intracellular Ca²⁺ level, and activity of iNOS in BPA treated RBL-2H3 cells but also the results indicated that the L. plantarum L67 extract inhibits expression of cytokines related to allergy such as TNF-α and IL-4 on the degranulation stage of mast cell. The L. plantarum L67 extract can inhibit to occur allergy caused by environmental hormone such as BPA.