We hypothesized that enhancing luteal tissue (or progesterone) would improve conception rate in Nili-Ravi buffaloes. The objective of the present studies were to standardized the dose of human chorionic gonadotropin (hCG) for ovulation induction on 7 d post insemination (experiment 1) and to see the effect of standardized dose of hCG, GnRH and exogenous progesterone (CIDR insert) on ovulation and conception rates in Nili Ravi buffaloes (experiment 2). In experiment 1, ovaries of the adult Nili Ravi buffaloes were scanned and mapped 7 d post synchronized estrus to confirm the presence of corpus luteum (CL) and dominant follicle (>8 mm) and assigned randomly to treatments of saline, or 500, 1000, 1500, 2000, 2500, or 3000 IU hCG (IVF-C, LG, Korea; i.m., n = 47) and ovulation induction or accessory CL, was confirmed a week later with ultrasound. Ovulation incidence in response to ≥2500 IU hCG was more (100%) than that of other doses or saline. The minimum effective dose was determined through dose response curve. In experiment 2, ovarian responses and conception rates were assessed by minimal effective dose of hCG (2500 IU, n = 58), GnRH (n = 28), CIDR insert for a week (1.38 g, n = 26) and control (n = 44) on 7 d post AI in previously CIDR synchronized in Nili-Ravi buffaloes. Overall significance was determined with Chi-squared and association between groups was checked using Z test in SAS software. More buffaloes were induced to ovulate with hCG (95%) and GnRH (80%) when compared with control and CIDR insert (P < 0.05). Conception rates were significantly higher (P < 0.05) in hCG (62%; 36/58) treated buffaloes compared with control (32%; 14/44). In contrast, these were non-significant in GnRH (50%; 14/28) and CIDR (42%; 12/26) treated buffaloes when compared with control. In conclusion 2500 IU of hCG is sufficient to induce the accessory CL and this treatment enhance the conception rate when given 7 d post insemination in Nili-Ravi buffaloes.