The objective was to determine the effects of graded concentrations of wheat bran (WB) on apparent ileal (AID), apparent total-tract (ATTD), and hind gut digestibility of nutrients and to test the effects of time for collecting fecal grab samples on nutrient digestibility in pigs. Six barrows (initial mean BW of 70.7 ± 5.7 kg) surgically fitted with a T-cannula in the distal ileum were used. A replicated 3 × 3 Latin square design was used with 3 diets, 3 periods, and 6 pigs. A basal diet was prepared to mainly contain wheat, soybean meal, and cornstarch. Two additional diets were formulated to contain 20 or 40% of WB at the expense of cornstarch. Each experimental period consisted of a 7-d adaptation period and a 4-d collection period. After the adaptation period, fecal samples were collected on d 8 and 11 using a grab sampling method, and ileal digesta samples were collected on d 9 and 10. To compare differences between nutrient digestibility calculated based on time points for fecal grab sampling, before or after the collection period for ileal digesta, a paired t-test was used. The AID of DM (81.8, 71.4, and 58.4%), OM (84.9, 75.0, and 62.4%), CP (78.2, 77.5, and 68.9%), and ADF (49.7, 45.4, and 36.1%) linearly decreased (P < 0.05) with increasing inclusion rate of WB from 0 to 40%. The ATTD of DM (91.8, 84.7, and 76.0%), OM (93.9, 87.2, and 78.8%), CP (89.7, 88.5, and 84.1%), and ADF (68.6, 53.4, and 39.1%) linearly decreased (P < 0.01) as the inclusion rate of WB increased. Hindgut digestibility of DM (9.6, 13.4, and 17.6%) and OM (8.7, 12.2, and 16.4%) linearly increased (P < 0.05), and that of NDF (30.5, 15.2, and 10.3%) and Ca (19.9, 18.2, and 10.1%) linearly decreased (P < 0.05) with increasing inclusion rate of WB. However, there were no differences in ATTD of nutrients between time points for feces grab sampling. In conclusion, the inclusion of WB reduced AID and ATTD of nutrients but increased hindgut digestibility of DM and OM, and there were no difference in the ATTD between the fecal collection time points of before and after ileal digesta collection in pigs.