Thirteen Pelibuey ewes (38 ± 7 kg) were used to evaluate the effect of replacing soybean meal (SBM) with a handmade product of fish (HMF). The experimental period lasted 6 weeks which comprised the last 3 weeks of gestation, where the change of body weight in pregnancy was individually evaluated, and the first 3 weeks of lactation, where changes in body weight, feed intake and milk production and composition were evaluated in sheep, and changes in body weight was evaluated on 22 of their kids (initial wt = 2.52. ± 0.05 kg). Control diet and the test diet used in both phases were formulated to be isonitrogenous and isocaloric. The level of inclusion of SBM and HFM in diets was 7 and 3.5%, respectively. HFM was elaborated with marine fish by-products (whole fish, heads, tails, and backbone). The chemical composition of HMF averaged: 50.8 ± 7.7% CP, fat 9.4 ± 0.8 and 22.7 ± 5.9% ash. There were no differences on DM intake, BW changes and milk production; however, as a result of an increase (<0.01) in the percentage of protein in milk with HFM treatment, was obtained greater final weight (19%, P < 0.01) of their suckling kids. It was concluded that HFM is a suitable substitute of SBM in gestating and lactating ewes. Replacing of SBM with HFM did not altered DM intake and milk performance in ewes. However, changes in the concentration of protein in milk in ewes that received HFM may be reflected in a greater weight gain of lambs in the first 21 d of age.