Dietary unsaturated fatty acids (FA) provide many challenges to ruminants as they are toxic to rumen microbes and result in formation of bioactive trans FA in the rumen that reduce milk fat yield. Corn silage and high moisture corn are commonly recognized as a risk factor for milk fat depression (MFD). The mechanism of increased risk of MFD is not clear, but may be due to the high concentration of linoleic acid (18:2) in corn. Corn silage and corn grain have a low concentration of FA, but due to their high inclusion rate in diets they contribute substantially to the polyunsaturated FA intake. Limited literature has reported the variation in FA profile of commercial hybrids as well as the contribution of individual plant parts to total FA in corn. To determine the location of FA in the corn plant, 4 stalks from 4 different commercial hybrids were separated into: stalk, husk + shank, leaves, cob, and kernels. On a DM basis, 80.3% of total FA were in kernels, 11.9% in leaves, 5.1% in the stalk, 1.7% in the cob, and 1.0% in husk + shank. More than 96% of the 18:1 and 92.4% of the 18:2 was in kernels, whereas 71.3% of the 18:3 was in the leaves. Second, we screened 67 corn silage and 36 corn grain hybrids from commercial test plots. Corn silage total FA (% of DM) averaged 2.54 ± 0.41 (mean ± SD, percentiles: 10th = 2.0 and 90th = 3.1). Corn silage 18:2 (% of DM) averaged 1.24 ± 0.24 (percentiles: 10th = 0.94 and 90th = 1.60) and 18:2 (% of FA) averaged 48.66 ± 3.0 (percentiles: 10th = 45.2 and 90th = 52.2). In the corn grains, total FA (% of DM) averaged 3.56 ± 0.27 (percentiles: 10th = 3.0 and 90th = 4.2), 18:2 (% of DM) averaged 2.05 ± 0.15 (percentiles: 10th = 1.8 and 90th = 2.4), and 18:2 (% of FA) averaged 57.5 ± 1.6 (percentiles: 10th = 53.4 and 90th = 60.8). In conclusion, moderate variation in 18:2 concentration (both % of DM and % of FA) was observed in commercially available hybrids. Corn FA profile may provide an additional tool to improve nutritional value of corn silage and other corn products for ruminants.