Abstract #T520
Section: Small Ruminant
Session: Small Ruminant II
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Gatlin Ballroom
Session: Small Ruminant II
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Gatlin Ballroom
# T520
Detection of gene expression and location of receptors activated by the oral administration of lithium chloride for conditioned taste aversion in sheep.
Katariina Vara1, Ahmed K. K. Salama1,2, Carmen L. Manuelian1, Maristela Rovai*1, Juan J. Loor3, Elena Albanell1, Xavier Such1, Gerardo Caja1, 1Group of Ruminant Research (G2R), Universitat Autònoma de Barcelona, Bellaterra, Spain, 2Animal Production Research Institute, Dokki, Giza, Egypt, 3Department Animal Science. University of Illinois, Urbana, IL.
Key Words: CNR1, GCG, food aversion
Detection of gene expression and location of receptors activated by the oral administration of lithium chloride for conditioned taste aversion in sheep.
Katariina Vara1, Ahmed K. K. Salama1,2, Carmen L. Manuelian1, Maristela Rovai*1, Juan J. Loor3, Elena Albanell1, Xavier Such1, Gerardo Caja1, 1Group of Ruminant Research (G2R), Universitat Autònoma de Barcelona, Bellaterra, Spain, 2Animal Production Research Institute, Dokki, Giza, Egypt, 3Department Animal Science. University of Illinois, Urbana, IL.
The emetic system activation (nausea and vomiting) plays a key role in the negative postingestive feedback. Lithium chloride (LiCl) is a nonlethal gastrointestinal drug that stimulates the chemoreceptor trigger zone, activating the emetic center and generating conditioned taste aversion (CTA) without other side-effects. There is scarce information on the pathways involved in the CTA mechanism by using LiCl. The aim of this study was to determine changes in gene expression of 4 genes (CNR1, GCG, GLP1R, HTR3A) involved in food intake and nausea. A total of 9 Manchega dry ewes, orally dosed with 225 mg LiCl/kg BW, were allocated into 3 euthanasia groups (0, 12 and 24 h after LiCl administration). Brain (area postrema), distal small intestine and colon tissue samples were collected, snap-frozen in liquid nitrogen, and stored at −80°C until analysis. The total RNA was extracted and purified using miRNeasy Mini Kit, and concentration and integrity determined by NonoDrop ND-1000 Spectrophotometer and Agilent 2100 Bioanalyzer, respectively. Gene expressions were determined by RT-qPCR with designed primers and 3 internal control genes. Data were normalized using the geometric mean of internal control genes, and expression values calculated using base-10 logarithm. As results, expressions of CNR1 and GLP1R were detected in all 3 tissues, and GCG was only detected in the distal small intestine and colon. The overall expressions of CNR1 and GLP1R differed by time and tissue, whereas GCG differed by time points. The CNR1 expression showed a linear regression in distal small intestine (decrease) and colon (increase) when comparing expression levels before and after Li administration. The GCG expression increased in distal small intestine and GLP1 expression decreased in colon 24 h after euthanasia. In conclusion, LiCl administration for a CTA treatment revealed changes in the expression of the genes CNR1, GCG and GLP1R in brain, distal small intestine and colon. Acknowledgments: Spanish Plan Nacional I+D+I (Project AGL2010–22178-CO2–01).
Key Words: CNR1, GCG, food aversion
