The objective of this study was to assess the suitability of using DNA methylation patterns in peripheral blood leukocytes as a marker of uterine function in the dairy cow. Peripheral blood and endometrial tissues were obtained at 29 d postpartum from cows with subclinical endometritis (SCE, n = 6) and control cows (CON, n = 6). DNA was extracted from peripheral blood leukocytes and endometrial tissues and DNA methylation was measured using Methylated DNA immunoprecipitation (MeDIP) in combination with microarrays. Genome-wide DNA methylation was assessed using a custom 400K Agilent microarray (GPL16270). The agreement between DNA methylation measured in leukocytes and in uterine tissue was assessed using Pearson correlation between log ratio in leukocytes and log ratio in intercaruncular and caruncular endometrium. Correlations were considered high if either of the 2 correlation coefficients was greater than 70%. All analyses were performed using SAS 9.2. Genes that were highly correlated between leukocytes and endometrial tissue were submitted for pathway enrichment analysis using PANTHER (Protein Analysis THrough Evolutionary Relationships). 9,733 DNA methylation probes had high (>70%) correlation between blood and endometrial tissue. These probes mapped to a total of 3,329 genes. Enrichment analysis identified several pathways of which the top 5 pathways were; gonadotropin releasing hormone receptor pathway (1.85 fold enrichment, P = 1.5 × 10⁻⁴), oxytocin receptor mediated signaling pathway (2.7 fold enrichment, P = 3.17 × 10⁻³), heterotrimeric G-protein signaling pathway-Gq α and Go α mediated pathway (1.9 fold enrichment, P = 4.2 × 10⁻³), PI3 kinase pathway (2.5 fold enrichment, P = 4.3 × 10⁻³), and Wnt signaling pathway (1.5 fold enrichment, P = 5 × 10⁻³). DNA methylation in the blood is highly correlated with DNA methylation status in endometrial tissue. In genes that were highly correlated, there was significant enrichment for several biological pathways that regulate the reproductive and immune systems. This study provides support for the efficacy of peripheral blood leukocytes as a marker of uterine function. Further research will establish if any of these markers are associated with improved reproductive function.