The use of various osmolyte products as a means for increasing blood glucose and mediating effects of shrink have been shown to be effective in different livestock species, but have been examined very little in goats. Therefore, the objective was to evaluate the effects of oral glycerol dosage on short duration transportation shrink in goats. On January 19, 2015, a total of 27 (23.4 ± 0.4 kg BW) intact and castrated male goat kids were stratified by sex and BW, and allocated randomly to 1 of 3 treatments: 1) control (C; n = 9); 2) 1 mL oral dose of glycerol per 0.45 kg of BW (n = 9); or 3) 2 mL oral dose of glycerol per 0.45 kg of BW (n = 9). Goats had ad libitum access to mixed-grass pasture and water until initiation of the study. At 0830, goat kids were gathered, bled, weighed, and dosed with glycerol according to treatment. Goats were then transported in a livestock trailer for approximately 4.5 h with an average ambient temperature of 12°C. After transport, goats were unloaded, bled, re-weighed, and returned to their pasture for the night. After a 24-h recovery time, goats were bled and weighed a final time. Data were analyzed using PROC MIXED of SAS with 2 preplanned orthogonal contrast statements: (1) the mean of control vs the mean of 1 mL and 2 mL, and (2) the mean of 1 mL vs 2 mL. Beginning weight, final weight, 24-h recovery weight, shrink, and recovery weight change did not differ (P ≥ 0.31) across treatments. Beginning white blood cell, final monocyte, basophil, and platelet, and 24-h monocyte counts were higher (P ≤ 0.05) and beginning neutrophil and eosinophil, and final red blood cell and white blood cell counts and mean corpuscular volumes tended (P ≤ 0.10) to be higher for 2 mL vs 1 mL. The 24-h neutrophil counts were higher (P = 0.05) and 24-h lymphocyte counts and white blood cell counts change tended (P ≤ 0.08) to be higher for C vs 1 mL and 2 mL. There were no differences (P ≥ 0.13) in the other blood measurements. Therefore, oral glycerol dosage may affect some blood measurements, but ultimately kid goats in this study did not shrink; thus, the effects of this treatment on short duration transportation shrink in goats were undetermined.