Two 21-d experiments were conducted to compare soybean meal (SBM) from high oleic to parent soybean cultivars on growth performance, ileal AA (IAA) digestibility, and apparent metabolizable energy (AME). Soybean meal was prepared by laboratory-scale mechanical extraction. In Exp. 1, growth performance was evaluated. Fifty 1-d-old male broilers (Ross 308) were randomly placed in battery cages and allocated to 2 dietary treatments with 5 replicates and 5 birds per replicate. The SBM sources consisted of cold-pressed conventional SBM (CON) and cold-pressed high oleic SBM (HO). All SBM sources were heated in a forced-air oven at 120°C for 20 min. Diets were formulated to meet or exceed NRC (1994) requirements. Broilers were weighed and feed disappearance measured on d 7, 14, and 21. In Exp. 2, IAA digestibility and AME were estimated using 100 one-d-old broilers randomly allotted to 3 dietary treatments with 10 replicates and 5 birds per replicate. Chicks were fed a corn-SBM based diet for 17 d, and a diet was developed by substituting the soybean cultivars at 48% in a corn starch-dextrose basal diet and fed from d 18 to 21. A nitrogen-free diet was fed to determine ileal endogenous AA losses. Titanium dioxide (0.5%) was included in all diets as a digesta flow marker. Excreta were collected from d 19 to 21 and ileal content at d 21. Statistical analyses were performed as a randomized complete block design using PROC GLM of SAS with significance level set at P ≤ 0.05. Chicks fed diets containing HO had decreased ADG (P < 0.05; 25.3 vs. 29.4 g) and increased feed conversion ratio (P < 0.05) from d 0 to 14 (1.71 vs. 1.56) and d 0 to 21 (1.54 vs. 1.47), compared with CON. Amino acid digestibility did not differ among all essential AA. There were no differences in AME (kcal per g) from SBM indirectly calculated between CON and HO, with values of 2,330 and 2,161, respectively. In conclusion, although there were no differences in nutrient digestibility, broilers fed HO had impaired performance compared with CON.