Nucleotides are not classified as essential nutrients as animals can synthesize them to a limited extend. However, studies have demonstrated their ability to improve biological responses including growth, immunity, and stress resistance. This experiment was conducted with a subset of sows in a commercial farm in China to evaluate the effects of late gestation and lactation supplementation with nucleotides and the performances of pigs post weaning. Sows (n = 40) around d 87 of gestation were randomly assigned to 2 dietary treatments: 1) the farm standard gestation and lactation diets (corn and soybean meal based,) or 2) the standard diets blended with 0.05% Ascogen (NT; Chemoforma, Switzerland) during gestation and 0.1% during lactation. At weaning, piglets (n = 112) from sows on each diet were split into 2 groups and fed diets that contained either 0 or 0.1% NT in a 42-d study which resulted in a 2 × 2 factorial arrangement. The data were analyzed by ANOVA using GLM program of SAS. Supplying NT to sow diets tended to reduce piglet mortality at birth (2.4 vs. 9.5%, P = 0.10) and the days to rebreeding (5.4 vs. 9.6d, P = 0.10). The individual piglet weight at birth and weaning as well as milk and serum immunoglobulin profile (IgA, IgG, IgM by ELISA) were not affected by NT. However, pigs from NT sows were heavier than those from control sows at Week 3 (13 vs. 12kg, P = 0.02) and Week 6 post weaning (22 vs. 20 kg, P = 0.01). They also had increased ADG (353 vs. 320 g, P = 0.01) and ADFI (592 vs. 539 g, P = 0.04) throughout the nursery period. Nursery treatments had no effect on growth performance. Piglets from NT sows had higher serum IgA levels at weaning (0.17 vs. 0.13 mg/mL, P = 0.01) and 1 week post weaning (0.20 vs. 0.17 mg/mL, P = 0.05), but no effect on serum IgG and IgM levels was observed. In conclusion, adding NT to the sow diets had beneficial effects on mortality of birth, days to rebreeding, and the growth of piglets during nursery in the commercial environment of this study. However, dietary NT supplementation in nursery may have no effect on growth.