Effect of trace mineral (TM) supplement source on cow performance and TM status over the production cycle were examined. Cows (n = 200, BW = 523 ± 6 kg, BCS = 5.1 ± 0.03) were divided in a factorial treatment arrangement, Angus (AN, n = 99) and Brangus, (BN, n = 101) and TM treatments (TRT; Inorganic = ING, n = 100, and Organic = ORG yeast source, n = 100). Supplementation (3 d/wk, 0.4 kg·⁻¹454 kg BW·⁻¹d in a pellet) of TM began ≥90 d before expected parturition and continued to weaning. A subset of cows had liver biopsies and serum collected, frozen and analyzed for TM content (Co, Cu, Fe, Mn, Mo, Se and Zn), by ICP-MS at 4 and 6 periods, respectively: start of TM (TMst), pre-calving (PreC), calving (C, serum only), post-calving (PostC, serum only), prebreeding (Pbrd) and weaning. Cow BW and BCS were recorded each period. Data were analyzed in SAS using Proc Mixed and repeated measures, with TRT, breed and time as fixed effects. No 3-way interactions were detected except for serum Cu, Mn and Mo. At PostC, BN were 0.17 BCS greater (P = 0.03) than AN. Cow BW and BCS did not differ (P > 0.21) by TRT at each period. The AN had greater (P ≤ 0.03) BW change from TMst to PreC (49 vs. 40 ± 2 kg) and from PostC to Pbrd (−44 ± 4 vs. −33 ± 3 kg) than BN. Change in BCS from PreC and Pbrd to weaning were greater (P < 0.05) in ING (−0.15 vs. −0.001 ± 0.05 and −0.36 vs. −0.10 ± 0.08, respectively) than ORG. Time (P ≤ 0.02) affected all serum and liver TM concentrations except for serum Fe (P = 0.13). Serum Co and liver Cu concentrations were greater (P < 0.05) in ING (1.7 vs. 1.3 ± 0.1 ng/mL and 266.4 ± 15.0 vs. 201.2 ± 14.1 µg/g, respectively), while serum Mo was greater (P = 0.006), in ORG (2.8 vs. 1.7 ± 0.3 vs. ng/mL). Serum Fe and liver Cu and Mn concentrations in BN (149.1 ± 5.5 µg/dL, 280.9 ± 15.2 and 11.7 ± 0.5 µg/g) were greater (P < 0.03) than AN (132.4 ± 5.3 µg/dL, 186.7 ± 13.9 and 9.5 ± 0.5 µg/g). Serum Se was greater (P = 0.003) in AN than BN (73.5 ± 1.7 vs. 65.4 ± 1.8 ng/mL). Cow TM status varies over time and is affected by breed and TM source; indicating development of nutritional management strategies can be based on cattle breed.