The effect cow prenatal trace mineral (TM) supplement source has on calf serum and liver TM status from 115 ± 21 d of age through weaning (206 ± 21 d) was investigated. Factorial arrangements [Angus = AN, n = 95 and Brangus = BN, n = 96 cows; Inorganic = ING, n = 98, and Organic = ORG yeast, n = 93] utilized calves born to cows supplemented (3 d/wk at a rate of 0.4 kg·⁻¹454 kg BW·⁻¹d in a pellet) with TM ≥90 d before expected parturition. All calf (n = 190) BW were collected at d 115 and weaning, while a subset of calves (ING, n = 12, 6/breed, ORG, n = 14, 7/breed) had liver biopsies performed and serum collected by jugular vein puncture. Processed serum and liver samples were frozen at −20°C until analyzed for TM (Co, Cu, Fe, Mn, Mo, Se and Zn), by ICP-MS. Data were analyzed in SAS using Proc Mixed and repeated measures, with TRT, breed and day as fixed effects. With the exception of serum Co, Mn and Mo, there were no (P ≥ 0.08) 3-way interactions. Calf d 115 BW (149 ± 2 kg) were not affected (P = 0.65) by TRT. At weaning, ORG had greater (P ≤ 0.02) BW (226 vs. 216 ± 3 kg), 205 d adjusted BW (227 vs. 216 ± 3 kg), BW gain (190 vs. 181 ± 3 kg) and ADG (0.93 vs. 0.89 ± 0.01 kg) than ING. Serum Co and Zn were affected (P < 0.001) by day, while all liver TM except for Co were affected (P < 0.03) by day. The ORG calves had greater (P ≤ 0.01) serum Se (29.8 vs. 18.2 ± 1.1 ng/mL), liver Se (0.7 vs. 0.5 ± 0.1 µg/g) and Mn (9.3 vs. 7.8 ± 0.4 µg/g) than ING. The AN had greater (P ≤ 0.04) serum Co (0.8 vs. 0.5 ± 1.2 ng/mL), Se (27.9 vs. 19.4 ± 1.1 ng/mL) and liver Co (0.2 vs. 0.1 ± 0.02 µg/g), Fe (573.5 vs. 435.9 ± 1.1 µg/g) and Zn (128.1 vs. 116.7 ± 1.0 µg/g), while BN had greater (P < 0.05) serum Mo (5.0 vs. 2.5 ± 0.6 ng/mL) and liver Mn (9.1 vs. 8.0 ± 0.4 µg/g). Calf BW and ADG at weaning were increased by prenatal ORG TM. These data indicate calf TM status varies by day relative weaning, prenatal TM source and breed. Additional research is needed to determine how TM source affects calf immune function, performance, carcass traits and reproductive potential.