The effect of cow prenatal trace mineral (TM) supplement source on calf TM status from birth through 30 d of age was examined. Factorial treatment (TRT) arrangements (Angus = AN, n = 95 and Brangus = BN, n = 96 cows; Inorganic = ING, n = 98, and Organic = ORG yeast, n = 93) utilized calves born to cows supplemented (3d/wk at a rate of 0.4 kg·⁻¹454 kg BW·⁻¹d in a pellet) with TM ≥90 d before expected parturition. Calf BW were collected at birth (n = 191). A subset of calves (n = 43) had BW, serum and plasma collected by jugular vein puncture at birth (0 h, before colostrum intake), 12 h, 24 h and 30 d of age. Processed samples were frozen at −20°C and plasma was refrigerated at 4°C until analyzed for TM (serum: Co, Cu, Fe, Mn, Mo, Se, Zn; plasma: Se), by ICP-MS. Data were analyzed in SAS using Proc Mixed and repeated measures, with TRT, breed and time as fixed effects. Breed, TRT, nor interactions affected (P > 0.34) BW at birth (36 ± 0.4 kg) and 30 d (69 ± 1.1 kg). With the exception of plasma Se, there were no (P ≥ 0.39) 3-way interactions. Time affected (P < 0.02) all 0–24 h serum and plasma TM except for Co (P = 0.60). Calf Mn concentrations were undetectable at 0–24 h, but were 1.9 ± 0.2 ng/mL at 30 d sample. Both serum Se (ING = 57.5 ± 1.7 and 44.7 ± 1.1 µg/mL vs. ORG = 46.5 ± 1.7 and 33.4 ± 1.2 µg/mL) and plasma Se (ING = 149.4 ± 3.2, 129.9 ± 3.2 vs. ORG = 118.7 ± 3.3, 95.4 ± 3.3 ng/mL) were affected (P < 0.001) by TM source at 0–24 h and 30 d, respectively. At 30 d, Co was greater (P = 0.002) for ING (2.0 ± 0.3 ng/mL) than ORG (0.6 ± 0.3 ng/mL). At 0–24 h, Fe and Se were greater (P ≤ 0.05) in AN (87.1 ± 5.9 µg/dL and 54.7 ± 1.7 ng/mL) than BN (70.1 ± 5.8 µg/dL and 49.2 ± 1.7 ng/mL). At d 30, AN (40.7 ± 1.2 ng/mL) serum Se was greater (P = 0.048) than BN (37.4 ± 1.1 ng/mL). In contrast, BN had greater (P < 0.01) Cu (0.3 vs. 0.2 ± 0.01 µg/mL) than AN at 0–24 h and Mo (52.9 ± 8.5 vs. 10.0 ± 1.7 ng/mL) at 30 d, respectively. These data demonstrate calf TM status varies by time, prenatal TM source and breed. Pre-calving nutritional strategies need to consider TM source and calf breed.