~~Multiparous cows (n = 18) were used in a randomized complete block design to study endocrine profile and hepatic gene expression related to IGF system on the adaptation to grazing after TMR feeding during early lactation. During the first 60 d postpartum (DPP), cows were assigned to 1) TMR (30 kgDM/d offered; 45% forage, 55% concentrate) and 2) 50% pasture in one (am) grazing session (6 h; pasture allowance 4 cm above ground level = 15 kgDM/d) + 50% TMR (15 kgDM/d offered) (G1). At 61 DPP and during 21 d, all cows were managed as G1 group determining 2 treatments (TREAT): PostTMR and G1. Plasma and liver biopsies were collected pre (+55 DPP) and post (+75 DPP) dietary change. Gene expression was quantified by SYBR-Green real time RT-PCR. Means from a repeated analysis using a mixed model were considered to differ when P < 0.05. Average milk energy output and BCS from 61 to 82 DPP did not differ between TREAT (27.5 and 25.7 ± 1.4 Mcal/d and 2.8 and 2.7 ± 0.1 units for PostTMR and G1, respectively). Plasma IGF1 concentrations at +75 DPP were greater in PostTMR than G1 cows (161 vs. 127 ± 26 ng/mL) as decreased from +55 at +75 DPP only in the latter cows. Plasma insulin and leptin as well as hepatic IGF1, IGF2 IGFBP2, and IGFBP4 mRNA were not affected by TREAT, DPP or its interaction. Before the diet change (+55 DPP), PostTMR cows had greater hepatic IGFBP1 mRNA but lower IGFBP3 mRNA than G1 cows. However, IGFBP1 mRNA had a 2.1-fold decrease while IGFBP3, IGFBP5 and IGFBP6 mRNA had 0.4 to 0.9-fold increase in PostTMR cows with the dietary change (+55 to +75 DPP). In addition, hepatic IGFBP3 mRNA decreased by 65% during this period in G1 cows. These changes determined that at +75 DPP, hepatic IGFBP3, IGFBP5 and IGFBP6 mRNA were greater while IGFBP1 mRNA was less in PostTMR than G1 cows. Decreased plasma IGF1 and hepatic IGFBP3 mRNA in G1 cows were associated with the increased milk yield from +55 to +75 DPP in these cows. In addition, while milk yield, BCS, and plasma IGF1 were maintained after the diet change in PostTMR cows, expression of IGFBP mRNA in the liver was modified in these cows