The creatinine excretion through the sampling spot of urine has been studied as an indicator of urinary volume. However, no studies in the literature have reported its use in animal grazing. The aim of this study was to evaluate the excretion of creatinine in beef cattle. Five heifers with 300 ± 15 kg average body weight were distributed in three 5 × 5 Latin squares and kept in individual paddocks equipped with feeding and drinking troughs. The experimental treatments were defined to represent those commonly used in the dry season, as follows: control (mineral salt), concentrated with 20.31% crude protein (CP) on dry matter (DM) being offered (OF) level of 0.5 to 1% of body weight fasted (BWF) OF5 and OF10, respectively; and 2 concentrated self-regulating (SR) consumption, containing 69.38% CP on a DM basis (20% urea and 20% salt) offered ad libitum (SR70) and other concentrate containing 39.73% CP based on MS being offered ad libitum (SR40). The experiment included four 17-d periods; the first 12 d were for adaptation, and the remaining 5 d were for data collection. The total collection of urine was carried out between d 13 and 17 of each experimental period, for which a Foley catheter number 26 2-way was used, with 50-mL flask placed on the d 13 of each experimental period. At the free end, the probe was coupled by conductive polyethylene hose to 2 L collection bag (polyethylene bag) with secured with ropes in a sturdy cotton fabric bag to the animal's neck in the ventral region. The animals were led to a weigh crate every 2 h from 0800 to 2000 h, and every 4 h from 2000 to 0800 h to facilitate the emptying of the bag. A urine sampling was performed every 4 h, obtaining a sample of 10 mL diluted in 0.036 N H₂SO₄. The creatinine content was assessed through an kinetic colorimetric test. Data were analyzed using the PROC MIXED procedure of SAS 9.1. The creatinine excretion was not affected by treatments or day and time of sampling (P > 0.05) with an average rate of 23.03 ± 0.30 (22.73 to 23.33) mg/kgBW. The spot sampling technique may be used to estimate the daily excretion of creatinine in animals kept in a pasture system.