Milk freezing point depression (FPD) content is regarded as being relatively stable in cows’ milk during lactation. Since the 30s, its measurement is a means to determine if water has been added to the milk. However, results from commercial herds have suggested that changes in milk FPD occurred during the year. The reason of those variations is unclear. However, dairy cow feeding might be an explanation. The objective of this experiment was to compare the characteristics of milk (especially milk FPD measured by MIR spectrometry) based on low input grass-based system compared with corn silage-based system, across 2 breeds of dairy cows (Holstein vs. Normande). Sixty-four dairy cows were observed from calving to drying off. Two feeding systems were compared. The Intensive system was designed to maximize individual performance, with a high energy diet (in winter, corn silage with 30% concentrate; in spring, summer and autumn periods, pasture with 4 kg/d of concentrate supplemented with corn silage from July). The Grass system was designed to decrease inputs (in winter, conserved grass with no concentrate; in spring, summer and autumn, pasture with no concentrate). The experimental design was a continuous design. Data were analyzed by using SAS mixed procedure. There was no significant effect of breed and feeding system on milk FPD. There was a significant effect of month of sampling and month of lactation. Milk FPD did not change from calving to 7th month of lactation. After that date, it largely decreased. These effects could be related to the high temperature in summer, specific feeding (grazing), or restricted access conditions for watering. There was also a significant effect of rank of lactation. FPD was higher for multiparous cows. This difference could be due to the opening of mammary tight junctions and a different repartition of ions and lactose on both sides of the epithelial barrier. Surprisingly FPD did not correlate with lactose content the most important osmotic agent in mammary epithelial cells. Some investigations have to be done to explain the mechanisms of FPD and its physiological variations.