The objective of this study was determine the effects of feeding omega 6 (ω6) fatty acids (FA) during an extended period of dry period until early lactation on blood metabolites of dairy cows. Thirty Holstein cows were used in 4 experimental groups in randomized design. The animals were randomly distributed to the following diets: Groups −90, −60, −30 and 0, supplemented with ω6 FA source (12% of whole raw soybeans – WRS, on DM basis) 90,60 and 30 d before expected calving date and from the calving date (0) until 90 d in milk. Diets were formulated according to NRC (2001) recommendations. Ether extract (EE) averaged 4.8 and 2.8% for diets containing WRS and without WRS respectively. The blood samples were taken on days −49, −35, −21, −14 and −7 of expected calving date, at calving and on d 7, 14, 21, 35 and 70 of lactation. Blood concentrations of glucose (GLU), total cholesterol (CHOL), very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL), triglycerides (TRI), total protein (TP), urea, gamma-glutamyl transferase (GGT) and aspartate aminotransferase (AST) were measured. Data were subject to PROC MIXED of SAS 9.3, diet were considered as fixed effect and time, interaction between diet and time, animal and residual error as random effect. The data were analyzed using polynomial regression using PROC REG of SAS 9.3. Linear decrease (P < 0.05) in GGT concentration and linear increase of TP and CHOL concentration were observed as the length of ω6 FA supplementation was longer. Quadratic effect (P < 0.05) was observed in AST blood concentration and the highest value was presented by cows of group −30 (86.4 U/L). Interaction effect (P < 0.05) was detected in GLU, TRI and VLDL blood concentration. The higher length of ω6 FA supplementation during pre-partum may improve hepatic metabolism of cows during transition period and early lactation.