Objective was to evaluate mRNA expression of genes associated with inflammation, oxidative stress, eicosanoid synthesis and transcription regulation in PMN and endometrium in cows receiving inorganic (INO) or organic (AAC) trace minerals. Twenty multiparous cows were allocated in a randomized complete block design, and received the same prepartum (1.5 Mcal/kg DM, 15% CP) and postpartum (1.76 Mcal/kg DM, 18% CP) diet. The diets were partially supplemented with an inorganic mineral mix of Zn, Mn, and Cu to supply 35, 45, and 6 ppm, respectively, of the total diet DM. Cows were assigned to an oral bolus administration containing a mix of either INO (n = 11) or AAC (n = 9) Zn, Mn, Cu, and Co to achieve 75, 65, 11, and 1 ppm, respectively, in total diet DM. Treatments began on d −30 and continued through d 30 relative to parturition. Organic trace minerals were supplied via AvailaZn, AvailaMn, AvailaCu, and CoPro (Zinpro Corp., Eden Prairie, MN). Endometrial biopsies were collected at d 14 and 30 postpartum. In PMN, expression of DEAD box polypeptide 58 (DDX58) was greater and Z-DNA binding protein 1 (ZBP1) was lower in cows supplemented with AAC. In addition, feeding AAC increased expression of genes related to oxidative stress (MPO) and eicosanoid metabolism (PLA2G4A, ALOX5AP). In endometrium, feeding AAC increased expression of genes related to oxidative stress (SOD1, NRF2), eicosanoid metabolism (PTGES) and inflammation (MYD88, STAT3, IL10). An interaction was observed for IL6 (inflammation) because expression increased from d 14 to 30 in cows fed INO compared with AAC. The decrease in IL1B, IL10, IL8 (inflammation) and PTGES (eicosanoids) expression on d 30 was more pronounced for cows fed AAC, whereas PPARG (an anti-inflammatory transcription regulator) increased on d 30. Overall, data demonstrate favorable alterations in PMN and endometrium inflammatory and oxidative status in cows supplemented with organic trace minerals.