Abstract #W417

# W417
Effects of wilting and additives on fermentation quality of alfalfa (Medicago sativa L.) silage.
H. Liu1,2, J. Q. Wang2, D. P. Bu*2,4, Z. W. Lv2, P. Sun2,3, 1College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China, 2State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China, 3Synergetic Innovation Center Of Food Safety and Nutrition, Harbin, China, 4CAAS-ICRAF Joint Lab on Agroforestry and Sustainable Animal Husbandry, Beijing, China.

The effect of wilting and different additives on alfalfa silage fermentation and nutritive value were studied under laboratory conditions. Lucerne (at the early bloom stage of maturity), wilted for 3 and 12 h respectively, was ensiled at 27.4% and 39.2% dry matter (DM) contents. Chopped grasses at each moisture content were either untreated (control) or treated with (1)inoculant (LAB) containing Lactobacillus plantarum LP70, Lactobacillus casei LC05, and Enterococcus faecium EF08 (a final application rate of 2 × 105 cfu/g of fresh forage); (2)sugar beet pulp (SB) at 50g kg−1 of fresh forage weight; (3)sodium formate (SF) at 6g kg−1 of fresh forage weight; (4)a combination of inoculant and sugar beet pulp (LAB+SB), and (5)sodium formate and sugar beet pulp combined (SF+SB). Forages were packed into triplicate 1-L laboratory silos per treatment at a density of 650 g/L. Silos were opened and analyzed after 65 d of ensiling. The results showed that all treatments had lower pH and lower concentrations of acetic and ammonia-N (of % TN), but higher concentrations of lactic acid than did untreated silages (P < 0.001). These effects were stronger in the LAB+M-treated silage. Addition of LAB+M resulted in silage with the highest concentrations of lactic acid (5.12 ± 0.36; P = 0.011) and the lowest ammonia-N (1.87 ± 0.07; P < 0.001) over treatments. Treated silages had similar concentrations of neutral detergent fiber (NDF), acid detergent fiber (ADF) and dry matter recovery compared with untreated silage. The addition of sugar beet pulp (SB) greatly reduced crude protein (CP) concentrations (20.53 ± 0.12 vs. 21.57 ± 0.12; P < 0.001) compared with control. Water-soluble carbohydrate concentrations were found increased in silage from alfalfa herbage treated with LAB+SB (2.09 ± 0.04 vs. 1.80 ± 0.04; P < 0.001) and SF-treated silage (2.04 ± 0.04 vs. 1.80 ± 0.04; P < 0.001) compared with untreated silage. Wilting reduced the rate of decline of silage pH (P < 0.001) and produced silages with lower acetic acid (P < 0.001) and ammonia-N contents (P < 0.001). As silage wilting was increased, silage quality was improved. Inoculant and sugar beet pulp combined (LAB+SB) was found to be the most effective additive.

Key Words: wilting, additive, alfalfa silage