Abstract #167
Section: Ruminant Nutrition
Session: Ruminant Nutrition: Dairy rumen fermentation
Format: Oral
Day/Time: Monday 11:00 AM–11:15 AM
Location: Panzacola G-1
Session: Ruminant Nutrition: Dairy rumen fermentation
Format: Oral
Day/Time: Monday 11:00 AM–11:15 AM
Location: Panzacola G-1
# 167
Effect of 3-nitrooxypropanol on ruminal fermentation, methane and hydrogen emissions, and methane isotopic composition in dairy cows.
Laiz F. de Matos1, Michael T. Harper1, Juliana Lopes*1, Fabio Giallongo1, Joonpyo Oh1, Danielle Gruen2, Alexander N. Hristov1, Maik Kindermann3, Stephane Duval4, 1Department of Animal Science, The Pennsylvania State University, University Park, PA, 2Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA, 3DSM Nutritional Products, Animal Nutrition and Health, Basel, Switzerland, 4DSM Nutritional Products France, Research Centre for Animal Nutrition and Health, Saint Louis Cedex, France.
Key Words: methane, 3-nitrooxypropanol, archaea
Effect of 3-nitrooxypropanol on ruminal fermentation, methane and hydrogen emissions, and methane isotopic composition in dairy cows.
Laiz F. de Matos1, Michael T. Harper1, Juliana Lopes*1, Fabio Giallongo1, Joonpyo Oh1, Danielle Gruen2, Alexander N. Hristov1, Maik Kindermann3, Stephane Duval4, 1Department of Animal Science, The Pennsylvania State University, University Park, PA, 2Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA, 3DSM Nutritional Products, Animal Nutrition and Health, Basel, Switzerland, 4DSM Nutritional Products France, Research Centre for Animal Nutrition and Health, Saint Louis Cedex, France.
The objective of this crossover experiment was to investigate the effect of a methane inhibitor, 3-nitrooxypropanol (3NOP), on rumen fermentation and enteric CH4 emission in lactating dairy cows. Six ruminally-cannulated late-lactation (235 DIM; SD = 20 d) Holstein cows were assigned to 2 treatments: control and 3NOP (60 mg/kg DMI). Each experimental period consisted of 10 d for adaptation and 4 d for sample collection. Compared with the control, 3NOP decreased (P < 0.001) CH4 emission by 31% (487 vs. 335 g/d, respectively) and increased (P < 0.001) that of H2 from 0.005 to 1.33 g/d. CH4 emissions per kg of DMI or milk yield were also decreased (P < 0.001) 34 and 37%, respectively, by 3NOP. The isotopic composition of CH4 was similar between treatments: control, δ13CCH4 = −20.91 ± 0.32‰, δDCH4 = −266.92 ± 0.14‰, and Δ13CH3D = −1.96 ± 1.78‰; and 3NOP, δ13CCH4 = −24.91 ± 1.72‰, δDCH4 = −266.94 ± 0.27‰, and Δ13CH3D = −1.72 ± 2.97‰. Concentrations of total VFA and propionate in ruminal fluid were not affected by treatment. Acetate concentration tended to be lower (P = 0.08) and acetate:propionate ratio was lower (P < 0.001) for 3NOP compared with the control. Butyrate and isovalerate concentrations tended to be or were increased (P ≤ 0.08) by 3NOP. Methanogenic archaea (Methanobrevibacter, Methanosphaera, and Methanomicrobium) were not affected (P ≥ 0.46) by 3NOP. Prevotella spp., the predominant bacterial genus in ruminal contents (22 to 23% of the total isolates), was also not affected (P = 0.54) by 3NOP. Compared with the control, Ruminococcus and Clostridium spp. were decreased (P ≤ 0.03) and Butyrivibrio spp. was increased by 3NOP: 8.2 vs. 6.5%, 6.2 vs. 4.1%, and 3.6 vs. 4.8%, respectively. This experiment demonstrated that a substantial inhibition of enteric CH4 emission in dairy cows resulted in increased H2 emission and decreased acetate concentration, but had no effect on rumen archaea. The isotopic composition of CH4 was similar between the 2 treatments, supporting the conclusion that there was little to no change in the metabolic strategy of the rumen archaeal population.
Key Words: methane, 3-nitrooxypropanol, archaea
