Fecal starch content has utility to estimate starch digestibility in dairy herds. We evaluated the relationship of total-tract starch digestibility and fecal starch content in individually fed lactating Holsteins. The data set from 5 experiments conducted at the Better Nature Research Center had 190 cow/observations fed at ad libitum intake. Starch content of feces, orts, and feeds were analyzed enzymatically at the Federal University of Lavras laboratory (Hall, 2009). Fecal production was measured by total collection of feces in buckets by trained personal. Feces were collected concurrent to defecation during 3 8 h sampling periods and weighed. The second and third sampling periods begun 8 h later than the previous sampling, to avoid a major disturbance to the animals while still representing a 24-h collection period. Fecal aliquots (equal fresh weight basis) were immediately frozen during the collection period and a composite sample was formed. Diets contained 37 to 42% whole plant corn silage in DM and mature finely ground corn grain and/or high moisture corn at 17 to 26% of DM. Cows BW, DMI, and milk yield (MY) during the period of fecal sampling were obtained. Mean ± SD (Min-Max) were: BW 636 ± 73 kg (500–860), DMI 20.74 ± 2.93 kg/d (11.88–27.83), MY 31.16 ± 6.22 kg/d (13.70–47.20), DMI/BW 3.28 ± 0.45% (2.07–4.57), MY/DMI 1.51 ± 0.25 (0.70–2.41), fecal starch content 5.09 ± 2.42% of DM (1.05–13.08), starch intake 6.021 ± 0.985 kg/d (3.374–8.356), consumed diet starch content 29.06 ± 2.84 (22.74–37.72), starch digestibility 94.96 ± 2.60% of intake (86.24–99.18). The correlation coefficient (r) of starch digestibility and fecal starch content was −0.924, higher than with starch intake (−0.219), diet starch content (0.136), DMI (−0.362), DMI/BW (−0.376), MY (−0.363), and MY/DMI (−0.121). Based on stepwise procedure, Starch digestibility (% of intake) = 100.02 – 0.9945 x fecal starch content (% of DM) (r² = 0.854, P < 0.01). Model R² was 0.878 when diet starch content was included and 0.882 when MY was further added (0.15 significance required for entry into the model). Fecal starch content was a good predictor of starch digestibility for this data set of cows sampled on similar experimental condition and fed corn based diets.