Abstract #W355

# W355
Do the viability and dose of Saccharomyces cerevisiae affect the digestibility, ruminal fermentation, and performance of lactating dairy cattle?
Y. Jiang*1, R. M. Martins2, I. M. Ogunade1, M. A. Bamikole3, F. Amaro2, W. Rutherford4, S. Qi4, F. Owens4, B. Smiley4, K. G. Arriola1, C. Staples1, A. T. Adesogan1, 1Department of Animal Science, University of Florida, Gainesville, 2Animal Science Department, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil, 3Department of Animal Science, University of Benin, Benin City, Nigeria, 4DuPont Pioneer, Johnston, IA.

This study was conducted to examine the effect of the dose and viability of supplemental Saccharomyces cerevisiae on ruminal fermentation and performance of lactating dairy cows. Four ruminally cannulated lactating cows (284 ± 18 DIM) were assigned to 4 treatments arranged in a 4 × 4 Latin square design with four 21-d periods. Cows were fed a non-acidotic total mixed ration (46.8% corn silage, 8.5% wet brewers grain and 44.7% concentrate, dry matter basis). The diet was supplemented with no yeast (Control, CON) or with a low dose of live yeast (5.7 × 107 cfu/day; LLY), a high dose of live yeast (6.0 × 108 cfu/day; HLY), or a high dose of killed yeast (6.0 × 108 cfu/day before heating at 80°C; HDY). Ruminal pH and temperature were measured with indwelling probes. Ruminal fluid was collected 0, 2, 4, 6, 8 and 10 h after the morning feeding on d 21 for analysis of volatile fatty acids (VFA) and ammonia-N. All data were analyzed using the GLIMMIX procedure of SAS (SAS Institute, Cary, NC). In vivo digestibility was measured using chromic oxide as a marker. Supplemental LLY increased yields of milk (29.9 vs 31.8 kg/d; P = 0.04), milk fat (1.09 vs 1.17 kg/d; P = 0.08) and milk protein (0.95 vs 1.02 kg/d; P = 0.05), feed efficiency (0.85 vs 0.90; P = 0.03), acetate to propionate ratio (P = 0.07), and in vivo apparent digestibility of dry matter and neutral and acid-detergent fiber (P = 0.02, 0.02 and 0.02, respectively). Feeding HLY instead of LLY decreased milk yield (31.8 vs 30.1 kg/d; P = 0.07) and feed efficiency (0.90 vs 0.86 kg/d; P = 0.05). Supplementing with killed instead of live yeast decreased concentrations of acetate (P = 0.06), propionate (P = 0.05) and total VFA (P = 0.05). Supplementing with HDY instead of CON increased lactate (P = 0.14) concentration and acetate to propionate ratio (P = 0.08) and decreased the minimum ruminal pH (P = 0.10). The low dose of live yeast increased dry matter and neutral-detergent fiber digestibility, milk production and feed efficiency but the high dose of killed or live yeast did not.

Key Words: yeast, dairy cow, milk yield