Abstract #161
Section: Ruminant Nutrition
Session: Ruminant Nutrition: Dairy rumen fermentation
Format: Oral
Day/Time: Monday 9:30 AM–9:45 AM
Location: Panzacola G-1
Session: Ruminant Nutrition: Dairy rumen fermentation
Format: Oral
Day/Time: Monday 9:30 AM–9:45 AM
Location: Panzacola G-1
# 161
Methanogenesis reduction ability of monensin and essential oils from two Nigerian citrus species.
Musibau A. Bamikole1,2, Ibukun M. Ogunade*1, Felipe Amaro1, Yun Jiang1, Thiago F. Bernardes1, Darren D. Henry3, Vania R. Vasconcelos1, F. O. Ugiagbe2, U. J. Ikhatua2, Nicolas DiLorenzo3, Adegbola T. Adesogan1, 1University of Florida, Gainesville, FL, 2University of Benin, Benin City, Nigeria, 3North Florida Research and Education Center, University of Florida, Marianna, FL.
Key Words: essential oil, citrus, in vitro fermentation
Methanogenesis reduction ability of monensin and essential oils from two Nigerian citrus species.
Musibau A. Bamikole1,2, Ibukun M. Ogunade*1, Felipe Amaro1, Yun Jiang1, Thiago F. Bernardes1, Darren D. Henry3, Vania R. Vasconcelos1, F. O. Ugiagbe2, U. J. Ikhatua2, Nicolas DiLorenzo3, Adegbola T. Adesogan1, 1University of Florida, Gainesville, FL, 2University of Benin, Benin City, Nigeria, 3North Florida Research and Education Center, University of Florida, Marianna, FL.
The effects of essential oils from sweet orange (Citrus sinensis, EOS) and tangerine (Citrus tangerina, EOT) on rumen fermentation, methane production and digestibility were studied. A corn silage-based TMR (0.5 g; CP 16.6%; NDF 35.9%) was treated with EOT or EOS at rates of 0 (Control), 10 (Low), 20 (Med) and 30 (High) μL/50 mL of rumen fluid-buffer inoculum (ratio 1:2) and with monensin (0.6 mg/50 mL). Each suspension was incubated in a 120-mL gas-tight culture bottle in triplicate at 39oC for 24 h in each of 2 runs. Fermentation parameters, gas and methane production, in vitro DM digestibility (DMD), and fermentation efficiency (FE; DMD g kg−1/gas volume) were measured. Data for each EO were separately analyzed with the Glimmix procedure of SAS. Adding EOS or EOT did not depress DMD (g/kg; 541 and 548, respectively) but adding monensin did (555 vs. 526; P < 0.05). Gas volume (mL/g DM) was increased by Low EOS or EOT (84.5 vs. 92.8 and 96.3) and decreased by High rates or monensin (75.3, 73.6 and 66.8), respectively. Hence, FE was reduced by Low EOS or EOT (6.58 vs. 5.81 and 5.70) and increased by High rates or monensin (7.52, 7.43 and 7.92), respectively. Methane production (mg/g DM digested) was increased by Low EOS and EOT (11.4 vs. 14.4 and 15.3) and reduced by High rates and monensin (7.10, 7.10 and 6.58; decreases of 37.7, 37.7 and 42.2%), respectively. Ammonia-N concentration (mg/dL) was increased by Med EOS (21.43) and EOT (20.6) versus monensin (4.63) or Control (11.3). Ruminal pH was increased by monensin (5.66 vs 5.75) but not by EOT (5.63 – 5.72) or EOS (5.67 to 5.71). Total VFA concentrations (mmol) of EOS (54.7 to 69.2), EOT (68.24 to 121.8), monensin (71.0) and control (80.2) treatments did not differ (P > 0.05). Monensin decreased acetate molar proportion and increased that of valerate, and Low EOT reduced the molar proportion of propionate. High doses of EO from Citrus sinensis and Citrus tangerina reduced in vitro methane production without adverse effects on feed digestibility and VFA production. By comparison, monensin reduced methane production and digestibility.
Key Words: essential oil, citrus, in vitro fermentation
