Abstract #272

# 272
Effects of inoculated lactic acid bacteria on aflatoxin B1 in corn silage.
Zhengxin Ma*1, Felipe X. Amaro1, Juan J. Romero1, Adegbola T. Adesogan1, 1Department of Animal Sciences, University of Florida, Gainesville, FL.

The objective was to determine the effect of adding viable and unviable silage inoculant bacteria on binding of aflatoxin B1 (AFB1) and silage quality. A corn hybrid was harvested at 35% DM, chopped to achieve a theoretical length of 1.9 cm and treated in quadruplicate with deionized water (Con) or 30 µg/kg of AFB1, or with a mixture of AFB1 and viable, heated or acid-treated forms (1 × 109 cfu/g) of Lactobacillus plantarum R2014 (Lp), L. buchneri R1102 (Lb), or Pediococcus acidilactici EQ01 (Pa). Each of the inoculants was incubated for 1 h in 30 mL of phosphate-buffered saline (pH 7.3) at 37°C (viable) or 85°C (heated) or in 30 mL of 0.003 M HCl (pH 2.5) at 37°C (acid-treated) before application. Silages (3 kg) were stored for 21 d in polythene bags. Concentrations of AFB1 were measured after 0, 24, 48, and 72 h of ensiling and silage samples from d 21 were analyzed for chemical composition, pH, volatile fatty acids and lactate. The experiment had a completely randomized design. Data were analyzed using the GLIMMIX procedure of SAS (SAS Institute Inc., Cary, NC). The AFB1 concentration decreased linearly (P < 0.05) to 0.35 µg/kg or less within 3 d of ensiling across treatments. Bacterial inoculation did not (P > 0.05) increase the AFB1 reduction. Treatment with AFB1 reduced (P = 0.03) the CP concentration (8.83 vs. 8.25%) and increased (P < 0.001) butyric acid concentration (0.37 vs. 0.62%) but inoculation prevented (P < 0.05) these effects. Treatment with viable Lp increased (P < 0.05) the acidification of the forage in the first 3 d and the lactic acid concentration on d 21 (1.52 vs. 3.97%). However, inoculation with viable Lb or Pa did not (P > 0.05) affect the chemical composition, suggesting that typical beneficial effects of these bacteria were attenuated by AFB1. The spiked aflatoxin was reduced to a safe level within 3 d of addition. Inoculation with bacteria did not increase AFB1 removal but viable Lp prevented the increase in butyric acid and decrease in CP caused by the toxin.

Key Words: aflatoxin B1, lactic acid bacteria, silage