The objective of this study was to evaluate the oral supplementation of Trp and pyridoxine to nursing piglets on performance and behavior after weaning. Forty 8 piglets (24 males and 24 females) from 8 sows were used. At d 14, 6 piglets per sow were divided into 3 treatments (2 animals/treatment) and were allotted in a randomized block design with 8 replicates. Treatments consisted of a Control (alanine + glucose + distilled water); Trp (3.5 g Trp/day + distilled water); Trp + pyridoxine (3.5 g Trp/day + 0.0095 g pyridoxine/day + distilled water). Each treatment was orally supplied 5 times/d and each dose contained 2.5 mL, totalling 12.5 mL/d. The supplementation was carried out during a 7 d period (d 14 to d 21 of age), and at d 21 piglets were weaned. After weaning, piglets from the same treatment were housed together in nursery pens and all animals, from each treatment, received the same pre-starter diet. During nursery period (d 21 to d 34) piglets were monitored in 3 different periods (at d 22, from d 28 to d 29 and from d 33 to d 34 of age) by a set of cameras. Behavior (standing, lying, eating, socializing, sitting and drinking) was assessed by an instantaneous sampling that was performed with intervals of 5 min to obtain the frequencies (%) of each behavior. Eating behavior was considered when pigs were eating at the feeders. Piglets’ performance was evaluated in the nursing and nursery period. Piglets receiving oral Trp + pyridoxine showed higher body weight (P = 0.06) on the first day after weaning (d 21 to d 22) compared with the Control which probably affected the weight loss (P = 0.06) of piglets on the first day after weaning, which was lower for piglets that received oral Trp + pyridoxine. No changes in performance were observed in the remaining periods. From d 28 to d 29 piglets that received Trp supplementation in nursing period, with or without pyridoxine, showed a higher eating behavior (P = 0.01) than the Control, which probably affected eating behavior (P < 0.01) in the total period (from d 21 to d 34). It can be concluded that oral supplementation of Trp and its association with pyridoxine, one week before weaning, reduces the weight loss in the first day after weaning and increases eating behavior until 1 wk after weaning.