Methane (CH₄), a greenhouse gas, can be held responsible for loss of 2–12% the gross energy of the diet. The objective of this study was to evaluate the effect of enzymes on ruminal metabolism and the production of enteric CH₄ in cattle. Five cows with average weight of 923.04 ± 86.76 kg were assigned to 5 treatments in 5 × 5 Latin square design: (1) diet without enzymes (control); (2) diet with 7.5 g/animal/day of amylase (Amaize, Alltech); (3) diet with 15 g/animal/day of xylanase (Fibrozyme, Alltech); (4) diet with 7.5 g/animal/day of cellulase + protease (Allzyme VegPro PO, Alltech); (5) Diet with 30g/ animal/day of enzymes mixture (amylase, xylanase, cellulase + protease). Animals were fed twice daily at 0800 and 1600 h. Each experimental period consisted of 21 d, and collections were made in the last 6 d. On d 21, ruminal pH was measured every 10 min using a continuous measurement device and rumen contents were collected at 0, 3, 6, 9 and 12 h after feeding to determine the production of short chain fatty acids (SCFA) and CH₄ by ex situ technique. Data were analyzed using SAS, through the MIXED procedure. The model included the effect of treatment as fixed factors, animal and period effects as random factors. The addition of different enzymes in the cattle diet showed no significant difference (P > 0.05) in relation to dry matter intake. There were significant differences (P < 0.05) for SCFA production in relation to the control diet. The enzyme mixture improved the production of acetic acid, propionic acid and total SCFA, while protease + cellulase improved the production of propionic acid. The CH₄ production was not significantly difference among treatments (P > 0.05). Also no significant differences (P > 0.05) were observed for the pH variables, as minimum, average and maximum pH, as well as time and area whiche pH was below to 5.8, 6.0, 6.2 and 6.5. It is concluded that these additives did not affect the variables DMI, CH₄ production and pH. The association of the enzymes showed improvement in the production of rumen SCFA without increasing the emission of CH₄.