Abstract #W282

Section: Production, Management and the Environment
Session: Production, Management and the Environment III
Format: Poster
Day/Time: Wednesday 7:30 AM–9:30 AM
Location: Gatlin Ballroom

# W282
Enteric methane mitigation and evaluation ruminal parameters of cattle fed cottonseed and vitamin E.
Ricardo Galbiatti Sandoval Nogueira^*1, Flavio Perna¹, Eduardo Cuellar Orlandi Cassiano¹, Lizbeth Collazo Paucar¹, Mariane Cheschin Ernandes¹, Diana Carolina Zapata Vasquez¹, Adrielle Matias Ferrinho¹, Romulo Germano de Resende¹, Felipe Bispo Mendonça¹, Renata Gardenalli¹, Angélica Simone Cravo Pereira¹, Paulo Henrique Mazza Rodrigues¹, ¹University of São Paulo, Pirassununga, São Paulo, Brazil.

Objective this study was verify the enteric methane emissions and evaluate ruminal parameters caused by the inclusion of 30% of cottonseed (diet with 8,32% ether extract) and 0,4% vitamin E (500 IU) in the diet of cattle. Six cannulated cows were distributed in a replicate 3x3 Latin square. Treatments were (1) control: basal diet; 2) CS: basal diet plus 30% cottonseed and 3) VitE: basal diet plus 30% cottonseed plus 0,4% vitamin E. Ruminal fermentation ex situ (micro-rumen) technique, in which, a sample (10 mL solid and 20 mL liquid) of rumen content was collected via cannula and put inside a sealed glass flask and incubated for 30 min at 39°C, starting samples removed the vials analyzed for the enteric methane emissions, total short chain fatty acids production (SCFA) and energy loss on methane compared with other products of rumen fermentation (REL). Results were compared through orthogonal contrast, where contrast 1: CS and VitE vs control; contrast 2: CS vs VitE. Data analyzed for SAS 9.3 and was considered 5% significance level. Results are shown in Table 1. Cottonseed inclusion reduced methane emissions. Production of propionate was similar between treatments, in average 82.76 g kg d⁻1. Cottonseed inclusion reduced the acetate, butyrate, SCFA and acetate propionate ratio (C2:C3). REL was similar between treatments, in average 29.45%. Include 30% of cottonseed in cattle diet reduces rumen fermentation, resulting in lower enteric methane emissions and total short chain fatty acids production. Vitamin E does not affect ruminal fermentation products. Table 1. Effect of including cottonseed and vitamin E on the production of SCFA, methane, and REL

Variable	Control	CS	VitE	SEM	C1	C2
Acetic, g kg d⁻¹	210.1	143.9	133.9	11.8	0.0028	0.7091
Propionic, g kg d⁻¹	80.94	85.35	82.02	5.51	0.8017	0.7910
Butyric, g kg d⁻¹	90.72	51.36	44.30	4.22	0.0001	0.3883
SCFA total, g kg d⁻¹	381.8	280.6	260.2	19.6	0.0045	0.6411
C2:C3	3.607	2.193	2.413	0.17	0.0097	0.6139
Methane, g kg d⁻¹	43.79	26.40	24.13	1.42	0.0001	0.3584
REL	31.31	29.67	27.35	1.36	0.4623	0.5980

C1 = CS and VitE vs. control; C2 = CS vs. VitE.

Key Words: lipids, energy loss on methane, short-chain fatty acids