Healthy nutrition is a preoccupation of most of the world’s population, thus the importance of animal science studies to improve milk nutrient composition. The aim was to study the vitamin E and selenium with sunflower oil (SFO) added to the diet of lactating cows to improve the nutrient profile of milk. Twenty-eight cows were allocated in 4 treatments, as follows: C (control diet); O (4% of SFO in dry matter (DM) diet); A (3,5 mg/ kg DM of organic selenium + 2000 IU of vitamin E/cow per day);OA (4% of SFO in DM diet + 3,5 mg/ kg DM of organic selenium + 2000 IU of vitamin E/cow per day). Cows were fed with 0.50 of concentrate, 0.42 of corn silage and 0.08 of coast-cross hay (DM). Blood and milk were taken in the last week of trial and analyzed for selenium and α-tocopherol. Data were analyzed as a RCBD with a factorial treatment structure (GLM/SAS). The addition of selenium and vitamin E in the cow’s diet increased selenium and α-tocopherol serum (0.083, 0.085, 0.337 and 0.389 μg/L, P < 0.0001, 0.022 SEM of selenium and 4.51, 6.26; 6.92 and 8.97 mg/dl, P = 0.0009, 0.66 SEM of α-tocopherol for C, O, A and OA, respectively) and milk (0.011, 0.027, 0.235 and 0.358 μg/L, P < 0.0001, 0.033 SEM of selenium and 2.27, 1.56; 3.08 and 2.89 mg/L, P = 0.0088, 0.36 SEM of α-tocopherol for C, O, A and OA, respectively). SFO supplementation increased α-tocopherol in cow’s blood (P = 0.0097). The antioxidants added to the diet of lactating cows improved the nutrient profile of milk. Financial support: FAPESP.