Abstract #W318
Section: Ruminant Nutrition
Session: Ruminant Nutrition: Dairy III
Format: Poster
Day/Time: Wednesday 7:30 AM–9:30 AM
Location: Gatlin Ballroom
Session: Ruminant Nutrition: Dairy III
Format: Poster
Day/Time: Wednesday 7:30 AM–9:30 AM
Location: Gatlin Ballroom
# W318
Milk yield and composition of Holstein cows fed increasing levels of amylolytic enzyme.
Caio Seiti Takiya*1, Gustavo Delfino Calomeni1, Thiago Henrique Annibale Vendramini1, Thiago Henrique Silva1, Guilherme Gomes Silva1, Jessica Cristiane Bertoni1, Carlos Eduardo Cardoso Consentini1, Rodrigo Gardinal1, Jefferson Rodrigues Gandra2, José Esler Freitas3, Francisco Palma Rennó1, 1Departamento de Nutrição e Produção Animal da Universidade de São Paulo, Pirassununga, São Paulo, Brazil, 2Faculdade de Ciências Agrárias da Universidade de Grande Dourados, Itahum, Mato Grosso do Sul, Brazil, 3Departamento de Zootecnia da Universidade Federal da Bahia, Ondina, Bahia, Brazil.
Key Words: α-amylase, Aspergillus oryzae, starch
Milk yield and composition of Holstein cows fed increasing levels of amylolytic enzyme.
Caio Seiti Takiya*1, Gustavo Delfino Calomeni1, Thiago Henrique Annibale Vendramini1, Thiago Henrique Silva1, Guilherme Gomes Silva1, Jessica Cristiane Bertoni1, Carlos Eduardo Cardoso Consentini1, Rodrigo Gardinal1, Jefferson Rodrigues Gandra2, José Esler Freitas3, Francisco Palma Rennó1, 1Departamento de Nutrição e Produção Animal da Universidade de São Paulo, Pirassununga, São Paulo, Brazil, 2Faculdade de Ciências Agrárias da Universidade de Grande Dourados, Itahum, Mato Grosso do Sul, Brazil, 3Departamento de Zootecnia da Universidade Federal da Bahia, Ondina, Bahia, Brazil.
The objective of this study was to evaluate the effects of increasing doses of amylolytic enzyme in diet of dairy cows on milk yield and composition. The enzyme used comes from Aspergillus oryzae extract enriched with α amylase activity (Amaize, Alltech Inc., Nicholasville, KY). The extract is in powdered form and was offered hand mixed into concentrate. Twent-four multiparous Holstein cows (average 120 DIM, 32 kg/d of milk yield and 580kg of live weight) were distributed in 4 × 4 Latin square design with 21-d periods and received the following treatments: (1) Control, without Amaize; (2) A150, diet containing 150 FAU/kg of Amaize (diet DM basis); (3) A300, diet containing 300 FAU/kg DM of Amaize (diet DM basis) diet; (4) A450, diet containing 450 FAU/kg of Amaize (diet DM basis). One FAU (fungal α-amylase unit) is the amount of enzyme which breaks down 1g starch in 1 h based on 11–25 min at pH 4.7 and 30°C. Experimental diet was formulated to achieve 30% of starch content. Corn silage was used as forage source and diet contained 48:52 ratio of forage and grain mixture. Cows were mechanically milked twice daily, and samples were collected proportionally from each milking on d 15, 16 and 17 of each experimental period. Milk samples were analyzed for fat protein and lactose (Milkoscan; Foss Electric, Hillerod, Denmark). Data were subjected to ANOVA and regression using PROC MIXED of SAS (2001). No differences were observed in milk yield and composition (Table 1). Inclusion of exogenous amylolytic enzyme did not improve milk yield and composition of mid lactating cows.
Table 1
10, 150, 300 or 450 FAU/kg (DM basis) of Amaize (Alltech Inc., Nicholasville, KY).
2Linear and quadratic effect.
| Item | Treatment1 | SEM | P-value2 | ||||
| 0 | A150 | A300 | A450 | L | Q | ||
| Milk yield, kg/d | 32.81 | 32.72 | 32.34 | 32.40 | 1.37 | 0.275 | 0.829 |
| 3.5% FCM, kg/d | 34.78 | 34.87 | 34.19 | 34.60 | 1.28 | 0.543 | 0.714 |
| Fat, kg/d | 1.26 | 1.28 | 1.24 | 1.26 | 0.05 | 0.594 | 0.917 |
| Protein, kg/d | 1.02 | 1.01 | 1.00 | 1.01 | 0.02 | 0.348 | 0.212 |
| Fat, % | 3.85 | 3.93 | 3.86 | 3.89 | 0.10 | 0.793 | 0.549 |
| Protein, % | 3.09 | 3.09 | 3.09 | 3.09 | 0.02 | 0.924 | 0.631 |
Key Words: α-amylase, Aspergillus oryzae, starch
