The objective of this study was to evaluate the effect of Se fertilization of ‘Jiggs’ bermudagrass (Cynodon dactylon L.) hayfields on the forage Se concentration and subequent Se status of calves consuming the forage. Sodium selenate was dissolved into water (8.8 g/L) and sprayed onto bermudagrass hayfields at a rate of 257 g Se/ha and harvested 8 wk after Na selenate application. Forage fertilized with Na selenate had greater (P < 0.001) Se concentration compared with the control forage without Se fertilization (7.7 ± 1.81 vs. 0.1 ± 0.04 mg/kg DM). Control and high-Se hay were fed for 42d to weaned calves (n = 32; initial BW = 176 ± 8.7 kg) stratified by initial BW and randomly assigned to partially covered drylot pens (16 pens; 2 calves/pen). Treatments were randomly assigned to pens, including control hay + high-Se hay, control hay + supplemental Na selenite, or control hay without supplemental Se (n = 7, 7, and 2 pens, respectively). A pair-feeding design was utilized, whereas each pen receiving high-Se hay was paired to a pen receiving Na selenite. Ground, high-Se hay was offered to assigned pens for a 4 h period each morning. Hay intake was measured and total Se intake was estimated. The Na selenite paired-pen was then provided the same daily amount of Se via Na selenite hand-mixed into a limit-fed grain supplement resulting in a range of daily Se intakes of 2.08 to 3.98 mg/d. Liver Se concentrations were greatest (P ≤ 0.004) on d 42 for calves consuming high-Se hay compared with calves receiving Na selinite or no supplemental Se (2.97, 2.49, and 0.60 mg/kg DM, respectively; SEM = 0.142). Amount of daily Se intake affected Se status, whereas, calves consuming <3.0 mg/d from high-Se hay had 54% greater (P = 0.02) liver Se concentrations compared with calves consuming the same amount of Se from Na selenite. When consuming >3.0 mg/d, there were no differences (P = 0.71) in liver Se concentrations due to Se source. These results imply that Se fertilization of bermudagrass hayfields results in increased Se concentration of harvested forage. Additionally, calves consuming this forage in amounts providing <3.0 mg Se daily have greater Se status compared with calves consuming the same amount of Se from Na selenite.