Polyspermic penetration as a result of in vitro fertilization (IVF) has shown to be a major obstacle in the production of porcine embryos. The objective of this study was to reduce the incidence of polyspermic penetration by supplementing coenzyme Q10 during oocyte maturation. Oocytes (n = 50/well) were maturated in tissue culture media 199 supplemented with 0, 10, 50 or 100 mM of coenzyme Q10 during the last 24 h of maturation. Frozen thawed semen from a single boar was used for fertilizing groups of approximately 30 oocytes in a concentration of 200 sperm/oocyte. Approximately 12 h after IVF, oocytes were evaluated for fertilization kinetics and subsequent embryonic development. A total of 600 oocytes over 3 replications were used in this study with one well/treatment/replicate. Data were analyzed using ANOVA with the main effects including treatment well and replicate. Chi-squared analysis was used to determine percentages of embryos reaching the different developmental states for each treatment. Oocytes supplemented with 50 mM coenzyme Q10 had significantly higher (P < 0.05) penetration rates (100 ± 14.97%) and pronuclear formation (MPN; 58.33 ± 19.54%) compared with 100 mM coenzyme Q10 supplementation. There was no difference between not supplementing coenzyme Q10 and 10 mM supplementation with respect to penetration and MPN formation rates. There was no difference between the treatments groups when considering the incidence of on polyspermic penetration. Cleavage at 48 h after IVF and blastocyst formation at 144 h after IVF were evaluated. Supplementing 100 mM coenzyme Q10 during oocyte maturation significantly decreased (P < 0.05) the percentage of embryos cleaved by 48h after IVF (3.33 ± 19.06%) and the percent of embryos at the blastocyst stage of development by 144 h after IVF (0.00 ± 15.76%) compared with all the other treatment groups. There was no difference between the other treatment groups with respect to cleavage and blastocyst formation rates. These results indicate that 50 mM coenzyme Q10 supplementation during oocyte maturation will not be detrimental to IVF and embryo culture in pig oocytes.