The study was performed in northern Mexico (25°64′ N, 103°26′ LW) to evaluate the effectiveness of different treatments to stimulate sexual behavior of rams during spring. Dorper rams (n = 20; 81 ± 0.32 kg BW; 3.8 ± 0.16 BCS) were divided in 4 homogeneous groups in terms of body weight (BW), body condition score (BCS) and odor, and were randomly assigned to 4 experimental groups: Glutamate Group (GG, n = 5; treated 7 mg kg⁻¹ BW of L-Glutamate i.m.), Testosterone Group (TG, n = 5; treated with 25 mg of testosterone i.m.), Glutamate + Testosterone Group, (GTG, n = 5; receiving 7 mg kg⁻¹ BW of L-Glutamate + 25 mg of testosterone i.m.) and Control Group (CONT, n = 5; which received 0.5 mL of saline i.m.). Odor score of rams was evaluated every 2 weeks by smelling the base of the horns at a distance of 15 cm and using a 0–3 scale. After 30 d of treatments, 2 males were randomly selected from each treated group and placed in one of 4 groups of anovulatory ewes (n = 14 each; 42 ± 2.36 kg BW; 3.04 ± 0.22 BCS) to evaluate the male sexual behavior, considering the appetitive sexual behavior (ASB), the consummatory sexual behavior (CSB) and isolation behavior (ISL), during 2-h daily x 2 d. While odor data were evaluated by ANOVA, sexual behavior considered X² (SYSTAT 12.0). No differences (P > 0.05) regarding odor score was observed in the first sample among experimental groups. Yet, at the end of the experimental period, the GTG showed the highest values (1.5 ± 0.29, P < 0.05) compared with groups GG and TG (0.75 ± 0.34 and 0.65 ± 0.75, respectively; P > 0.05). In the ASB, the GTG depicted the highest percentage of performed behaviors regarding GG, TG and CONT (43 vs 29, 11 and 18% respectively); only statistical differences (P < 0.05) occurred with respect to TG and CONT. In the CSB-phase, GG and GTG (37%) and the CONT (23%) did not differ (P > 0.05), yet, the TG depicted the lowest CSB performance (P < 0.05). In the case of ISL, the CONT accumulated the highest percentage (62%; P < 0.05) regarding GTG, GG and TG (25, 13 and 0%, respectively).Results demostrate that Dorper rams treated with glutamate + testosterone promoted not only an increased sexual behavior but also an augmented odor score during spring.