Abstract #620

# 620
Effects of rumen-protected methionine, lysine, and histidine on lactation performance of dairy cows.
F. Giallongo*1, J. Oh1, M. Harper1, J. Lopes1, A. N. Hristov1, H. Lapierre2, R. A. Patton3, I. Shinzato4, J. Tekippe4, C. Parys5, 1Department of Animal Science, The Pennsylvania State University, University Park, PA, 2Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC, Canada, 3Nittany Dairy Nutrition Inc, Mifflinburg, PA, 4Ajinomoto Co. Inc, Tokyo, Japan, 5Evonik Industries AG, Hanau, Germany.

The objective of this study was to evaluate the effects of rumen-protected (RP) Met, Lys and His supplementation to a metabolizable protein (MP)-deficient diet on performance of dairy cows. The experiment was a 9-wk randomized complete block design with 36 Holstein cows (DIM, 132 ± 30 d; BW, 611 ± 81 kg). After a 2-wk covariate period, cows were blocked by DIM, milk yield, and parity, and randomly assigned to 1 of the following 6 treatments: control [AMP; +245 g/d of MP over NRC (2001) requirements]; MP-deficient diet (DMP; −118 g/d of MP); DMP supplemented with RPMet (30 g/d of Mepron; Evonik Industries AG; DMPM); DMP supplemented with RPLys (130 g/d of AjiPro-L; Ajinomoto Co., Inc.; DMPL); DMP supplemented with RPHis (120 g/d of an experimental product; DMPH); and DMP supplemented with RPMet, RPLys and RPHis (DMPMLH). The AMP and DMP diets consisted of (DM basis): 42% corn and 21% alfalfa silages and 37% concentrates and contained 16.5 and 14.5% CP, respectively. DMI tended to be decreased (P = 0.07) by DMP compared with AMP (28.0 vs. 29.4 kg/d). Milk and energy-corrected milk yields were decreased (P < 0.03) by DMP (40.5 and 36.1 kg/d) vs. AMP (44.1 and 42.1 kg/d). Milk protein content was increased (P ≤ 0.03) by DMPH and DMPL (3.17 and 3.20%) compared with DMP and AMP (3.01%), and tended (P = 0.06) or was numerically higher (P ≤ 0.15) for DMPMLH and DMPM (3.15 and 3.12%) vs. DMP and AMP. Milk fat content was decreased by DMP vs. AMP (3.33 and 3.90%; P = 0.04) and was increased by DMPH and DMPMLH (3.93 and 4.01%; P ≤ 0.03) compared with DMP. Yields of milk protein and milk fat were decreased (P = 0.01) by DMP vs. AMP (by 10 and 20%, respectively). Cows fed AMP had higher MUN (11.7 mg/dL; P < 0.01) compared with cows fed the DMP diets (on average 8.09 mg/dL). Overall, feeding an MP-deficient diet decreased DMI and yields of milk, protein, and fat. Addition of RPAA to the DMP diet generally increased milk protein content but did not affect protein yield. Supplementation of RPHis alone or in combinations with RPMet and RPLys also increased milk fat content.

Key Words: methionine, lysine, histidine