Abstract #W194

# W194
Effect of bacterial additives on fermentation quality and aerobic stability of rye silage harvested at dough stage.
Young Ho Joo*1, Seong Shin Lee1, Dong Hyeon Kim1, Hyuk Jun Lee1, Sardar M. Amanullah1,3, Ouk Kyu Han2, Sam Churl Kim1, 1Division of Applied Life Science (BK21Plus, Inst. Agric. & Life Sci.), Gyeongsang National University, Jinju, Gyeongsangnam-do, South Korea, 2National Institute of Crop Science, RDA, Suwon, South Korea, 3Bangladesh Livestock Research Institute, Dhaka, Bangladesh.

This study was carried out to determine the effect of different additives on chemical composition, fermentation characteristics and aerobic stability of rye silage harvested at dough stage. Rye forage was grown at National Institute of Crop Science, Suwon, South Korea, and harvested at 37% of dry matter. The harvested forages were divided into the following 4 treatments: CON (distilled water at 2 mL/kg of fresh forage); LP (L. plantaruma t 1.5 × 104cfu/g of fresh forage); LB (L. buchneri at 1.2 × 104cfu/g of fresh forage) and MIX (mixture of LP and LB at 1:1 ratio). The forage was chopped to 3–6 cm length and ensiled into 10 L bucket silo with 4 replications for 0, 1, 4, 7, 48 and 100 d. On 100 d of ensiling, crude protein concentration (7.61 vs. 6.63%) in LP was higher (P < 0.05) than that in CON. The concentrations of neutral detergent fiber and hemicellulos were higher (P < 0.05) in LB (73.7 and 24.6%) and MIX (72.9 and 24.0%) than those in CON (70.4 and 21.7%) and LP (69.5 and 22.6%), while acid detergent fiber concentration was lowest (P < 0.05) in LP (46.9 vs. 48.7, 49.1 and 48.9%). The CON had higher (P < 0.05) pH (4.56 vs. 4.15, 4.11 and 4.12) than all treatments applied bacterial additives. The pH in LB and MIX silage decreased more drastically than those in CON and LP on early ensiling periods (d 0–7). The concentrations of lactate and propionate were highest (P < 0.05) in LP (13.4 and 5.72%), but acetate concentration was highest (P < 0.05) in LB (27.5%) and MIX (22.1%). Lactate to acetate ratio (1.23 and 1.43 vs. 0.29 and 0.35) was higher (P < 0.05) in CON and LP than that in LB and MIX. Lactic acid bacteria (6.71, 6.59 and 6.48 vs. 5.25 log10 cfu/g) in CON, LP and LB were higher (P < 0.05) than that in MIX, whereas aerobic stability (760.5 vs. 616.3, 606.5 and 634.3 h) was higher (P < 0.05) in MIX than all other treatments. Yeast was highest (P < 0.05) in LB (7.64 log10 cfu/g), while mold was not detected in all treatments. In conclusion, LP or MIX supplementation can improve the aerobic stability by increase of acetate concentration which has strong anti-fungal effect.

Key Words: aerobic stability, barley silage, inoculant