Abstract #622

# 622
Milk protein yield and plasma concentration of methionine increase in dairy cows fed field peas supplemented with rumen-protected amino acids.
Andre B. D. Pereira*1, Andre F. Brito1, Kayla M. Aragona1, Simone F. Reis1, Kelsey A. Juntwait1, 1University of New Hampshire, Durham, NH.

Previous studies showed that diets with more than 15% field peas (FP; DM basis) resulted in reduced milk and milk protein yield in dairy cows. We hypothesize that decreased yields were caused by limited supplies of MP-Lys and MP-Met due to extensive degradation of FP RDP in the rumen. The objective of this study was to evaluate diets with 25% of FP supplemented with rumen-protected (RP) Lys (AjiPro-L) and Met (Smartamine-M) as a substitute for corn meal (CM) and soybean meal (SBM). Twelve multiparous and 4 primiparous lactating Holstein cows were blocked by DIM, milk yield and parity, and randomly assigned to 1 of 4 diets in a replicated 4 × 4 Latin square design. Diets were 35.5% corn silage, 15.5% grass-legume haylage, 5.9% roasted soybean, and: (1) 36% CM and 1.3% urea (3.59:1 MPLys:MPMet ratio; negative control: NC), (2) 29.7% CM, 9.8% SBM, and RPLys-Met (3.07:1 MPLys:MPMet ratio; positive control: PC), (3) 25% FP, 12.3% CM, and 2.4% SBM (3.88:1 MPLys:MPMet ratio; PEAS), and (4) 25% FP, 12.2% CM, 2.4% SBM, and RPLys-Met (3.13:1 MPLys:MPMet ratio; PEASAA). Data were analyzed using the MIXED procedure of SAS and Tukey adjustment for means separation (α = 0.05). All dietary treatments had 15.1% CP and 1.58 Mcal/kg. As expected, cows fed NC had decreased DMI, milk protein yield and total concentration of ruminal VFA and increased excretion of MUN. Cows fed NC and PEAS had decreased plasma concentration of Met. Feeding cows PC, and PEASAA mitigated these negative responses. Results showed that plasma Lys was higher for diets fed FP, suggesting that Met is the first limiting AA in FP-based diets. Table 1. Feeding field peas to dairy cows
ItemDietSEMP-value
NCPCPEASPEASAA
DMI, kg/d23.30b24.96a24.63a25.00a0.39<0.01
Milk, kg/d41.7942.9342.6942.711.040.53
Milk fat, kg/d1.401.411.451.490.040.09
Milk protein, kg/d1.15b1.25a1.21ab1.24a0.03<0.01
MUN, mg/dL9.85c7.93a9.09b8.77b0.27<0.01
Ruminal total VFA, mM103.08c113.23b111.92b120.92a1.84<0.01
Plasma Lys, mM67.20b69.94b77.21a76.97a3.960.03
Plasma Met, mM19.92c27.71b19.63c31.89a1.06<0.01

Key Words: field peas, dairy cow, rumen-protected amino acid