Linseed meal antioxidants could help prevent oxidative degradation of omega-3 enriched milk. Six Holstein dairy cows (120 ± 30 DIM, 36.3 ± 6.5 kg milk/d; Mean ± SD) were used in a replicated 3 × 3 Latin Square design (20-d periods; 14 d of adaptation) investigating the effect of linseed meal on animal performance and oxidative stability of omega-3 enriched milk. Linseed oil was abomasally infused continuously to all cows at 243 ± 23 g/d, and dietary treatments were: 1) Linseed meal (16.5% of DM; LS), Canola meal (16.5% of DM) + 7000 units of vitamin E/kg DM (VE), Canola meal (16.5% of DM; CON). Milk yield was recorded and sampled for composition analyses on the last 3 d of each period. Oxidation measurements were done in fresh milk collected on d 17. Data were analyzed using the MIXED procedure of SAS (SAS 9.3, The SAS institute, Cary, NY) including the random effects of period and cow, and the fixed effects of square and treatment. Preplanned contrasts were CON vs. LS and VE vs. LS. Milk yield and fat corrected milk were not affected by treatment and averaged 34.2 ± 2.3 and 32.8 ± 2.3 (Mean ± SE), respectively. Milk fat concentration tended to be lower in LS (3.76%) relative to CON (4.00%; P = 0.06), but was no different from VE (3.73%). Milk protein concentration was higher in LS (3.38%) compared with CON (3.30% P = 0.01), and was not different from VE (3.38%). The concentration of lactose and the yields of fat, protei, and lactose were not different among treatments. The concentration of C18:3 n-3 was not affected by treatment and averaged 5.1 ± 0.79% of total milk fatty acids. Treatments had no effect on the concentration of dissolved oxygen, redox potential or conjugated diene hydroperoxides of fresh milk, which averaged 5.5 ± 0.17 mg/L of milk, 148.5 ± 24.1 mV and 0.92 ± 0.06 mmol/L, respectively. However, VE reduced milk concentrations of the volatile lipid oxidation products propanal, hexanal, and 1-octen-3-one by >73% (P < 0.05) and tended to reduce hept-cis-4-enal concentrations by 90% (P = 0.06) relative to LS, whereas there were no differences between LS and CON. Vitamin E may help prevent oxidative degradation of milk moderately enriched in omega-3, whereas linseed meal has no effect.