Nucleotides have demonstrated their ability to improve several biological responses during stress periods, including parturition. This experiment was conducted in a commercial farm (WENS Groups) in China. Around d 87 of gestation, sows (n = 316) were assigned to 2 dietary treatments based on their housing unit: 1) the standard farm gestation diets provided by WENS Group or 2) the standard diets top dressed with 3.5 g/sow/d of a nucleotide product (Harv-con NT, NT; Interflavor Ltd., China). Approximately 1 week before expected parturition, sows were moved to farrowing facilities and assigned to 2 dietary treatments: (1) the standard farm lactation feed; or (2) the standard diet top dressed with 8 g/sow/d (on the day of moving to 7 d post-farrowing) and 12 g/sow/d (7 d post-farrowing till weaning) NT in the lactation diet, which resulted in a 2 × 2 factorial arrangement. The data were analyzed using GLM program of SAS with the housing unit as the experimental unit, 4 units per treatment. Supplying NT to sow diets during late gestation had no effect on the total litter size at birth as expected (11.5 vs. 11.9, P = 0.19), but reduced the “healthy” pig litter size (9.9 vs. 10.8, P = 0.01) as determined by the farm. Litter weight of the healthy pigs at birth was not affected by the treatments (14.4 vs. 14.4, P = 0.88), thus the individual birth weight was increased in NT sows (1.4 vs. 1.3kg, P = 0.01). In the units with diarrhea, less diarrhea was observed in litters from sows fed NT in gestation compared with those from Control sows (25 vs. 69%, P = 0.07). Also, more sows in the gestation NT treatment returned to heat within 7 d post weaning compared with control sows (95 vs. 82%, P = 0.01). Neither NT treatment in gestation or lactation had an effect on colostrum and d13–17 milk nutrient composition (i.e., fat, protein, and lactose) or immunoglobulin profile. In conclusion, adding NT to the sow diets during late gestation increased healthy pig birth weight and ratio of returning heat within 7d post weaned. It also reduced diarrhea incidence during the suckling period in the units that experienced diarrhea. However, supplying NT to the sow diets during lactation had no effects on these observations. Supplying NT to sow diet during gestation or lactation had no effects on milk composition as well.