Some pre-dips leave residues in bulk tank after milking. Hydrogen peroxide is digested by lactoperoxidase, an enzyme released from the mammary gland during milking, which would mean this teat dip would not leave residues in the milk (Isobe et al., 2011). The objective of this study was to determine if hydrogen peroxide-based teat dip a (OxyCide, GEA, Naperville, IL) leaves a residue in the bulk tank milk after use as a pre-dip. The study was conducted using 97 Holstein cattle at the University of Kentucky Coldstream Dairy. Three samples were collected from the bulk tank 20 different times, 10 from the 3:30 a.m. and 10 from the 4:00 p.m. milking sessions; once at the beginning of milking, once during the middle, and once at the end of milking. The bulk tank is collected every other day so the dates of sampling were determined to have 10 a.m. and 10 p.m. samples before and after the bulk tank was emptied. The pre-dip was applied to the teats using teat dip cups. Then, 236.8 mL of milk from the bulk tank were collected into the same liquid measuring cup each sample. Peroxide test strips (Em Quant, Santa Ana, CA) were used to determine if hydrogen peroxide residues were present in the sample. In all samples collected throughout the study, the test strips tested negative for hydrogen peroxide residue. This study demonstrated that the enzymes in the milk successfully digested all detectable hydrogen peroxide from the pre-dip. This study suggests hydrogen peroxide can be used as a pre-dip to prevent any risk of residue left in the milk after milking.