Factors known to influence pregnancy rate in livestock species were evaluated in white-tailed deer. Pen-raised white-tailed does (n = 53) ranging in age from 1.5 yr to 6.5 yr were utilized to evaluate the effects of BCS, BW, disposition, and serum cortisol concentration on fawning rate to AI. All does received a CIDR for 14 d and an injection (i.m.) of 300 IU PMSG at CIDR removal. Laparoscopic insemination was conducted at approximately 60 h following CIDR removal. Prior to insemination, does were restrained in a drop-floor chute and were administered sedative. At this time, BW was collected and disposition scores ranging from 1 to 5 (1 = docile and 5 = aggressive) were assigned by independent observers to evaluate deer behavior as an indicator of stress while restrained in the chute. Immediately following insemination, BCS was evaluated and blood samples were obtained via jugular venipuncture to determine cortisol concentration by RIA analysis. Previous research indicated a moderately positive relationship (r = 0.30) between disposition score and serum cortisol concentration. Does were placed with cover bucks one week post AI. During fawning season, DNA samples were collected from all fawns to identify the sire of each fawn and determine fawning rate to AI. Data were analyzed using the LOGISTIC procedure of SAS. Pregnancy rate to AI was 67.9% and final pregnancy rate was 94.0% following natural service. Mean BCS was 3.02 ± 0.52 (range of 2 to 4), and mean disposition score was 2.16 ± 1.21 (range of 1 to 4). There was no significant effect of BCS (P = 0.59), BW (P = 0.19), cortisol concentration (P = 0.30), or disposition score (P = 0.64) on pregnancy rate to AI. Results of this study indicate that body condition and BW may not have as great of an influence on pregnancy rate in white-tailed deer as observed in livestock species. These data also indicate that the stress response to being restrained may not influence pregnancy rate to AI in white-tailed deer. Given the small sample size of this study, further research is needed to fully elucidate the influence of these factors on pregnancy rate to AI in white-tailed does.