The aim of this study was evaluated the gait score of broilers supplemented with vitamin D. For this, we used 2400 broilers distributed in a randomized blocks allocated in factorial design 2 × 2 × 2, being males and females, strains Cobb 500 and Ross 308, supplemented or not with vitamin D (25-OHD₃). The diets were formulated following the requirements of each phase and added to 69 mg of 25-OHD₃/t of feed in the supplemented treatments. After 41 d evaluated the gait score and, for this, 100% of the birds were put to walk in the path of a bounded linear meter inside the boxes. The way the birds walked was ranked scores ranging from 0 to 2, being gait score 0 bird that walked normally, gait score 1 bird which was difficult to walk and gait score 2 bird that walked with difficulty. We used the Chi-Square at a significance level of 5%. Females had higher (P < 0.05) frequency of gait score 0 (88.41%) and males had a higher frequency of gait score 1 (16.26%). The Cobb 500 strain of birds had higher (P < 0.05) incidence of gait score 0 (87.79%) when compared with the Ross 308 (83.10%). Supplementation with vitamin D (25-OHD₃) did not affect (P > 0.05) the gait score of birds. Therefore, it was concluded that vitamin D (25-OHD₃) did not improve the way of walking (gait score) of broilers.