Among the parasites with zoonotic potential, nematodes from the family Anisakidae are most involved in cases of infection due to the consumption of fish. The aim of this study was to evaluate the presence of parasites with zoonotic potential in Brachyplatystoma rousseauxii, Cynoscion leiarchus, Cichla spp., and Colossoma macropomum captured in the northern coast of Amazon/Brazil. During the months from January to December 2009, 60 specimens of each specie were weighed, measured, and identified according their anatomic traits. They were filleted and with the aid of a candling table, their muscles were evaluated for the presence of parasites. Nematode larvae were placed in Petri dishes containing distilled water and subsequently fixed in A.F.A. (95 parts of 70% alcohol, 5 parts of formalin, 2 parts of acetic acid) for 24 h. The nematodes identification was based in their morphologic traits. The amount of parasites in each specimen and overall were used to calculate the prevalence (P), infection intensity (I), mean infection intensity (MI) and mean abundance (MA). Nematodes of the genus Anisakis spp. were observed parasitizing 15 specimens of Cynoscion leiarchus P (37.50%), I (3–42), MI (18.06), MA (6.77). Three specimens exhibited multiple parasitism by Anisakis spp. and Poecilancistrium caryophyllum P (7.50%), I (1–2), MI (1.30), MA (0.10). Twelve specimens of Cichla spp. were infested, of which, 10 by nematodes of the genus Contracecum spp. P (17.00%), I (2–12), MI (2.50), MA (0.92) and 2 by Contracecum spp. and by Clinostomum complanatum P (17.00%), I (1–3), MI (1.70), MA (0.27). Nine specimens of B. rousseauxii were infested by Anisakis spp. P (15.00%), I (4–32), MI (12.00), MA (1.80). No parasite species were found in the 60 specimens of C. macropomum analyzed. The parasites found may be responsible for allergic and gastric reactions and may even lead to death in humans. The high prevalence of parasites with zoonotic potential combined with the small number of studies on the parasite fauna of the Amazon fish, represents a serious risk to human health, and reinforce the need for a larger number of studies in this area.