This study aimed to identify behavior and metabolites changes in piglets after different weaning when submitted to anxiety test. Thirty-two piglets were separated in 2 homogeneous groups, regarding sex and weight, within 2 pairs of sows based on same parturition date. Half of each sow litter was submitted to different weaning ages (days): 23 (early weaning - EW) and 30 (normal weaning - NW). One week after the NW date, piglets at the same age were submitted to an anxiety test (ATest) in elevate plus maze. Piglets were weighed and blood sampled on the day before and at weaning and also before and after the maze. Blood glucose (mg/dL), lactate (mg/dL) and protein (g/dL) concentrations were measured. ATest was recorded for 3 min and analyzed. GLM was run and means compared by Tukey’s test at 0.05. Average daily gain during the week after early weaning were greater (P < 0.01) for NW animals, that were still suckling, compared with EW animals. This scenario was inverted in the week after NW date. The blood glucose before weaning was greater (P < 0.01; SEM ± 2.26) for the EW (127.62) than for NW (114.28) piglets. Those differences were still present (P < 0.01; ± 3.11) in the blood before ATest for EW and NW at 115.92 and 101.54, respectively. Differences were not observed neither after weaning nor after ATest. Likewise, lactate was greater (P < 0.05; ± 4.48) in EW vs NW animals before weaning and ATest, with 53.55 vs 38.48 and 66.21 vs 35.09 (SEM ± 6.32), respectively. Inverted after weaning, where NW (52.85) animals presented greater (P < 0.05; ± 4.43) lactate compared with EW (39.87). Lactate did not change between groups after ATest. Total protein was different (P < 0.05; ±0.12) between EW and NW animals only after weaning, with 5.48 and 5.06, respectively. There was no difference between the EW and NW on the variables recorded in ATest. After anxiety test blood glucose was negatively correlated (−0.35; P < 0.01) to the entry in open arms. Early weaning changes blood parameters at weaning and when animals are submitted to novel environmental challenges. Blood glucose concentration is negatively associated with more active and less anxious animals in maze test.