Two breeding experiments conducted over a 2-year period were conducted to determine if conception rates in beef heifers and cows were affected in response to breeding facilities. The study compared the traditional squeeze chute and the insemination barn with timed artificial insemination programs. The heifer groups were given the 14 d CIDR-PG and cow groups the 7 d CO-Synch + CIDR protocol to determine whether conception rates are affected by insemination within a squeeze chute or insemination within an insemination barn. In Experiment 1, 17 beef heifers at the Barton Agricultural Research Center in Gordonville, Missouri were artificially inseminated following standard protocols. Cattle numbers were selected at random to determine which animals would be bred in the insemination barn or within the squeeze chute. Technicians were alternated so that each technician inseminated half of the animals in each facility setting. Ultrasound technology was used to determine which heifers were pregnant. Results indicated that 87.5% of heifers bred in the squeeze chute became pregnant as compared with 37.5% (P ≤ 0.05) of heifers bred in the insemination barn. Experiment 2 largely consisted of the same process. This time, however, 17 beef cows were inseminated. BioPryn (Moscow, ID) blood tests were used to confirm pregnancy within the cow population 30 d following insemination. Results indicated that 88.89% of the cows bred in the squeeze chute became pregnant whereas only 75% (P ≤ 0.10) in the insemination barn became pregnant. This indicates on average a 31.94% (P ≤ 0.05) higher rate of pregnancy from breeding in a traditional squeeze chute compared with the insemination barn. Future studies will include larger sample sizes of cattle and take into account technician preferences.