The objective of this study was to evaluate the effect of 2 nutritional strategies on age at puberty in Nellore heifers. Nellore cows were allocated to treatments by BW (438 ± 48 kg) and BCS (3.0 ± 0.5; 1 to 5 scale). The factorial experimental design (2 × 2) included 2 phases of supplementation: phase 1 – prepartum supplementation with protein (PPS; n = 73; 0.5 kg soybean meal/cow daily) during the last trimester of gestation or no supplementation (NPPS; n = 81); phase 2 – provision of creep-feed (creep diet was 22% CP, and 62% TDN) to calves for 118 d before weaning (CF; n = 78) or no creep-feeding (NCF; n = 75). After 7 mo of age (weaning), heifers were maintained in Brachiaria ssp. pastures. Heifers were weighed and reproductive status assessed by ovarian ultrasonography (detection of presence of CL) monthly from 14 to 30 mo old. The proportion of heifers that had attained puberty by 18, 26 and 30 mo of age, and BW were assessed using PROC MIXED and PROC GLIMMIX (differences with P < 0.05 considered significant). At weaning (6.4 mo), cow supplementation in last trimester of gestation did not affect BW of heifer calves (183 ± 1.9 vs. 184 ± 1.9 kg, P = 0.98 for PPS and NPPS cows, respectively). Creep feeding supplementation also did not influence BW at weaning (184 ± 1.9 vs. 183 ± 1.9 kg, P = 0.78 for CF and NCF, respectively). Prepartum supplementation did not change the proportion of heifers pubertal at 18 mo (4.1 vs. 6.2%, P = 0.53), 26 mo (18.5 vs. 16.4%, P = 0.57) and 30 mo (82.2 vs. 76.2%, P = 0.36 for PPS and NPPS, respectively) of age. Supplementation of suckled calves by Creep Feeding also did not change the proportion of heifers pubertal at 18 mo (3.8 vs. 6.7%, P = 0.43), 26 mo (15.8 vs. 19.1%, P = 0.77) and 30 mo (80.7 vs. 77.3%, P = 0.59 to CF and NCF, respectively) of age. In conclusion, neither the cow supplementation nor creep feeding used in the present study anticipated puberty in Nellore heifers.