Dipteran pests affect dairy calves through rapid transmission of pathogens and reduction in calf well-being. A feed-through larvicide may effectively manage fly pressure and improve well-being. The objective of this study was to determine the effect of pelleting a feed through larvicide on calf lying behavior. Holstein dairy calves (n = 12) were divided into 4 pens and assigned to one of 2 treatment sequences: control (CON)-washout (WAS)-larvicide (LAR) (n = 6) or LAR-WAS-CON (n = 6). CON and LAR lasted 4 d and WAS lasted 10 d. The control pellet was fed during CON and WAS and the experimental pellet was fed during LAR. Dataloggers recorded lying bouts and posture at 1-min intervals from d 0 to d 24. Fecal samples, collected on d 2, 4, 11, 20, and 14, were seeded with house (n = 25), stable (n = 25), face (n = 25), and horn fly eggs (n = 25) to assessed daily emergence of pupae and adults. Data were analyzed using PROC GLM of SAS to determine the effect of treatment period, sequence, and treatment period × sequence interaction. No effects on lying bouts were evident (P ≥ 0.26). Calves laid down longer during CON (17.1 ± 0.2 h/d) and LAR (17.2 ± 0.2 h/d) compared with WAS (14.4 ± 0.1 h/d; P < 0.0001). Further, calves laid down less frequently during WAS (11.6 ± 0.2 bouts/d) compared with CON (16.5 ± 0.4 bouts/d) and LAR (16.0 ± 0.4 bouts/d; P < 0.0001). Calves on the LAR-WAS-CON sequence had a tendency to stand longer (8.0 ± 0.2 h/d) than CON-WAS-LAR sequence calves (7.5 ± 0.2 h/d; P = 0.08). Fewer pupae emerged during the WAS period of the LAR-WAS-CON sequence (5.8 ± 1.5 pupae) compared with CON in CON-WAS-LAR (18.0 ± 1.0 pupae) and LAR-WAS-CON (19.1 ± 1.0 pupae), LAR in CON-WAS-LAR (18.8 ± 1.0 pupae) and LAR-WAS-CON (16.3 ± 1.0 pupae), and WAS in CON-WAS-LAR (13.7 ± 1.5 pupae; P < 0.0001). This indicates there may be a delayed response of larvicide. Further, fewer adults emerged during the LAR period (1.6 ± 0.8 adults) compared with the CON period (7.9 ± 0.8 adults) of the LAR-WAS-CON sequence (P < 0.01). These data suggest a pelleted feed through larvicide may be an effective fly management option for producers without negatively affecting calf behavior.