Abstract #320

# 320
Rumen-protected methyl donors during late pregnancy: 3. Maternal Smartamine M and its association with neonatal Holstein calf neutrophil gene network expression.
Carolina Bespalhok Jacometo*1, Zheng Zhou2, Erminio Trevisi3, Daniel Luchini4, Marcio Nunes CorrĂȘa1, Juan J. Loor2, 1Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil, 2University of Illinois, Urbana, IL, 3UniversitĂ  Cattolica del Sacro Cuore, Piacenza, Italy, 4Adisseo NA, Alpharetta, GA.

The aim was to evaluate the effect of supplementing pregnant cows with rumen-protected methionine (MET) on calf neutrophil expression of genes related to cell adhesion and chemotaxis, oxidative stress and inflammation. Forty Holstein calves born to cows receiving during the last ~4 wk of pregnancy MET (Smartamine M, Adisseo NA; ~2.9:1 Lys:Met; n = 20) or control (CON, ~3.35:1 Lys:Met, n = 20) were used. Immediately after birth calves were separated from the dam, fed first colostrum within 6 h (3.8 L with minimum IgG concentration of 50 g/L), housed individually and fed a common milk replacer (25% CP, 17% fat) twice daily. Blood neutrophils were isolated at birth (before receiving colostrum), 24 h after first colostrum and at 14, 28 and 50 (~1 wk post-weaning) d of age. Data were analyzed as repeated measures using the MIXED procedure of SAS. Neutrophil phagocytosis was not affected (P > 0.05) by maternal MET supplementation, but increased (P < 0.01) over time in both groups. Regardless of maternal diet SELL, CADM1, LCP1 and CYBA expression increased (P < 0.05) from birth to 24 h after colostrum intake, then decreased (P < 0.05) until 28 d. ZBP1 increased (P < 0.01) from birth to 28 d. SELL expression was overall greater (P = 0.04) in MET than CON calves. Expression of genes related to oxidative stress (MPO, NOS2, SOD1, SOD2, NFE2L2) was not affected (P > 0.05) by maternal diet. Similarly, blood biomarkers related to oxidative stress (ROMt, myeloperoxidase, retinol, tocopherol) were not affected (P > 0.05) by diet. TLR2 had lower (P = 0.04) expression in MET calves, but other inflammatory mediators (TLR4, MYD88, IRAK1, TRAF6, NFKB, TNF, IL1B, SLAMF7) and blood IL-1B and IL-6 concentrations were not affected (P > 0.05). A marked decrease (P < 0.01) in both cytokines from birth to 24 h after colostrum intake was observed regardless of diet. Overall, the data suggest that maternal supplementation with MET during the last ~4 wk of gestation had a minor effect on calf neutrophil gene network expression and blood biomarkers of oxidative stress and inflammation.

Key Words: fetal programming, nutrition, nutrigenomics