Abstract #318

# 318
Rumen-protected methyl donors during late pregnancy: 1. Maternal Smartamine M and its association with neonatal Holstein calf blood immunometabolic biomarkers.
Carolina Bespalhok Jacometo*1, Zheng Zhou2, Erminio Trevisi3, Daniel Luchini4, Marcio Nunes Corrêa1, Juan J. Loor2, 1Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil, 2University of Illinois, Urbana, IL, 3Università Cattolica del Sacro Cuore, Piacenza, Italy, 4Adisseo NA, Alpharetta, GA.

The aim was to evaluate the effect of supplementing pregnant cows with rumen-protected methionine (MET) on growth and blood biomarkers of hepatic and energy metabolism, inflammation and oxidative stress. Forty Holstein calves born to cows receiving during the last ~4 wk of pregnancy MET (Smartamine M, Adisseo NA.; ~2.9:1 Lys:Met; n = 20) or control (CON, ~3.35:1 Lys:Met, n = 20) were used. Immediately after birth calves were separated from the dam, fed first colostrum (3.8 L with minimum IgG concentration of 50 g/L), housed individually and fed a common milk replacer (25% CP, 17% fat) twice daily. Calves were bled at birth (before colostrum), 24 h after first colostrum, at 14, 28 and 50 (~1 wk post-weaning) d of age. Data were analyzed as repeated measures using the MIXED procedure of SAS. No maternal diet effect (P > 0.05) was observed in calf growth (body weight and withers height) from birth through weaning. MET calves had lower glucose at birth (4.05 vs. 4.73 mmol/L, P > 0.01), but there was no overall maternal diet effect (P = 0.18). Regardless of maternal diet, glucose, AST and GGT increased markedly (P < 0.01) from birth to 24 h after colostrum intake, then decreased (P < 0.01) at 14 d and remained unchanged until 50 d. NEFA and creatinine concentrations had a sharp decrease after birth (P < 0.01) while BHBA concentrations increased (P < 0.01) over time. Paraoxonase, albumin and ceruloplasmin concentration increased (P < 0.01) over time. MET calves had lower albumin (30.1 vs. 30.9 g/L, P = 0.09) and ceruloplasmin tended to be lower (1.58 vs. 1.85 μmol/L, P = 0.11). IL1-B and IL-6 had a marked decrease (P < 0.01) from birth to 24 h after colostrum intake. Tocopherol (1.31 vs. 2.19 µg/mL), myeloperoxidase (466 vs. 544 U/L) and ROMt (12.4 vs. 15.5 mg H2O2/100 mL) were lower (P < 0.05) in MET calves at 14 d of age. Retinol increased over time (P < 0.01). Overall, data suggest that maternal supplementation with MET during the last ~4 wk of gestation affected some biomarkers of metabolism and oxidative stress, hence, seemed to elicit a beneficial effect on the neonatal calf.

Key Words: dairy cattle, fetal programming, nutrition