Abstract #W70

# W70
Genome-wide association analysis and gene ontology enrichment of meat tenderness in Polled Nellore cattle in Brazil.
Leticia M. Castro1,4, Claudio U. Magnabosco2,3, Fernando B. Lopes2,4, Roberto D. Sainz*2,5, Guilherme J. M. Rosa6, 1Federal University of Goias, Goania, GO, Brazil, 2Embrapa-Brazilian Agricultural Research Corporation, Brasilia, DF, Brazil, 3CNPq-National Council for Scientific and Technological Development, Brasilia, DF, Brazil, 4Capes-Coordination for the Improvement of Higher Education Personnel, Brasilia, DF, Brazil, 5University of California, Davis, CA, 6University of Wisconsin, Madison, WI.

Brazil has the largest commercial cattle herd worldwide, but its meat quality is highly variable. The national herd is largely composed of Bos indicus breeds, which in general have less tender meat than Bos taurus cattle, decreasing the product value. This study was carried out to identify genomic regions and biological relevant pathways associated with meat tenderness in Polled Nellore cattle. Data consisted of Warner-Bratzler shear force (WBSF) values of Longissimus muscle after 7 d of aging, from 326 Polled Nellore animals born in 3 breeding seasons (2002, 2005 and 2009) at the OB ranch, located in the State of Mato Grosso, Brazil. The animals were genotyped using either the Bovine HD Chip (777k) or the GGP-Indicus Chip (77k). The imputation from the GGP to the HD Chip was performed using FImput software. SNPs were excluded when GenCall <0.7, Call rate <0.90, EHW P < 0.01 (using Bonferroni adjustment), and MAF <0.05. Due to large dispersion of sires (progenies’ parents), the population stratification was controlled by the 3 first genomic principal components. Genome-wide association analysis (GWAS) was performed using the Efficient Mixed-Model Association (EMMA) method. The GWAS was complemented with a gene set enrichment analysis of Gene Ontology (GO) terms using the FatiGO procedure. The most significant markers (P < 0.0001) were located on chromosomes 2, 3, 7, 10, 11, 17, 20, 21, 24 and 25, indicating several QTLs associated with meat tenderness throughout the genome. Additionally, 48 GO terms were deemed enriched. Several of these functional categories can be related to WBSF in Polled Nellore cattle, such as activities of ion channels, membrane cell transportation, growth factors, and protein serine/threonine phosphatase complex, which participate in processes that inactivate apoptosis components. These results help to elucidate the metabolic pathways related to this trait, which is of extreme economic and social importance to Brazil as Nellore is the dominant beef cattle breed in the country. Financial support: EMBRAPA, CNPq, CAPES.

Key Words: shear force, GWAS, pathway